MRI: Ultracentrifuge acquisition to support Bioengineering Red Fluorescent Tag for in vivo Microscopy

该提案所要求的超离心机是蓝藻研究表征和生物工程光敏蛋白的重要设备。有必要从大肠杆菌表达系统中分离用于生物工程蛋白的蛋白质。斯皮勒实验室的重点是设计一个小的，明亮的红色荧光蛋白标签在体内显微镜。米尔斯学院的本科生、研究生和二年级学生都从事实验室工作。他们在参与前沿研究的同时获得研究经验和专业知识。斯皮勒实验室的另一个目标是鼓励年轻女性从事科学事业。Spiller实验室在2010年4月获得了NSF-CBET-0967965的资助来资助这项研究。超离心机是这个工程的关键设备之一。斯皮勒实验室目前正在与加州大学戴维斯分校生物光子学科学与技术中心（CBST）合作开展一项前沿研究项目。他们正在使用PCR和大肠杆菌表达系统，从五(5)类（蓝/绿）蓝藻色素中捕获，表征和纯化GAF结构域。

The ultracentrifuge that is requested with this proposal is an essential piece of equipment for research characterizing and bioengineering light sensitive proteins from cyanobacteria. It is necessary to separate proteins from the E.coli expression system that are used for bioengineering proteins. The focus of The Spiller Lab is to engineer a small, bright red fluorescent protein tag for in vivo microscopy. Undergraduate, post baccalaureate and glide-year students at Mills College are engaged in the laboratory work. They gain research experience and expertise, while participating in leading edge research. Another goal of Spiller Lab is to encourage young women to pursue scientific careers. Spiller Lab was awarded a NSF-CBET-0967965 grant in April 2010 to fund this research. An ultracentrifuge is one of the critical pieces of equipment for this project. The Spiller Lab is currently working on a leading-edge research project in collaboration with Center for Biophotonics Science and Technology (CBST), University of California, Davis. They are using PCR, and E.coli expression system, to capture, characterize and purify GAF domains from the five (5) class II (blue/green) cyanobacteriochromes.