MRI: Ultracentrifuge acquisition to support Bioengineering Red Fluorescent Tag for in vivo Microscopy

这项提案所要求的超速离心机是研究蓝藻中光敏感蛋白的关键设备。有必要将用于生物工程蛋白的蛋白质从大肠杆菌表达系统中分离出来。斯皮勒实验室的重点是为活体显微镜设计一种小的、明亮的红色荧光蛋白标签。米尔斯学院的本科生、学士研究生和高年级学生都在从事实验室工作。他们在参与前沿研究的同时，获得研究经验和专业知识。斯皮勒实验室的另一个目标是鼓励年轻女性追求科学事业。斯皮勒实验室在2010年4月获得了美国国家科学基金会-CBET-0967965的拨款，用于资助这项研究。超速离心机是该项目的关键设备之一。斯皮勒实验室目前正在与加州大学戴维斯分校的生物光子学科学与技术中心(CBST)合作开展一项前沿研究项目。他们正在使用聚合酶链式反应和大肠杆菌表达系统，从五(5)类(蓝/绿)蓝藻碘铬中捕获、表征和纯化GAF结构域。

The ultracentrifuge that is requested with this proposal is an essential piece of equipment for research characterizing and bioengineering light sensitive proteins from cyanobacteria. It is necessary to separate proteins from the E.coli expression system that are used for bioengineering proteins. The focus of The Spiller Lab is to engineer a small, bright red fluorescent protein tag for in vivo microscopy. Undergraduate, post baccalaureate and glide-year students at Mills College are engaged in the laboratory work. They gain research experience and expertise, while participating in leading edge research. Another goal of Spiller Lab is to encourage young women to pursue scientific careers. Spiller Lab was awarded a NSF-CBET-0967965 grant in April 2010 to fund this research. An ultracentrifuge is one of the critical pieces of equipment for this project. The Spiller Lab is currently working on a leading-edge research project in collaboration with Center for Biophotonics Science and Technology (CBST), University of California, Davis. They are using PCR, and E.coli expression system, to capture, characterize and purify GAF domains from the five (5) class II (blue/green) cyanobacteriochromes.