FIBR: Planning: Proteomic Analysis of Critical Targets of Environmental Stress

FIBR：规划：环境压力关键目标的蛋白质组学分析

• 批准号: 0307044
• 负责人: Martin Feder
• 金额: $ 4.99万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-15 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0307044&HistoricalAwards=false
• 关键词: FIBR; Planning; Proteomic; Analysis; Critical

The proposed work will discover thermal "weak links" in organisms. That is, as organisms encounter progressively higher temperatures, which are the first proteins to be damaged by temperature? To do so, the work will exploit a molecular chaperone, a special protein that recognizes and binds to damaged proteins. These chaperones will be isolated - and with them the damaged proteins that they bind. The damaged proteins in turn will be identified by peptide mass fingerprinting, a novel, state-of-the-art technique. The proposed work is a pilot project that will seek weak links in populations of a model bacterial species, E. coli, that have been evolving at different temperatures, and test whether the weak links in low-temperature populations have been strengthened in high temperature populations.The proposed work will set the stage for a methodology whereby weak links can be identified for any organism whose genome has been characterized, and in relation to any natural or anthropogenic stress. Potentially these weak links determine the ecological and evolutionary limits of organisms, the maximum age they can reach, susceptibility to disease, etc. - but heretofore it has not been possible to identify the weak links themselves. The project will also be among the first to demonstrate the applicability of peptide mass fingerprinting to problems in this area of science.

这项工作将发现生物体内的热“薄弱环节”。也就是说，当生物体遇到越来越高的温度时，哪些蛋白质首先被温度破坏？为了做到这一点，这项工作将利用一种分子伴侣，一种特殊的蛋白质，可以识别并结合受损的蛋白质。这些伴侣蛋白会被分离出来，它们结合的受损蛋白质也会被分离出来。受损的蛋白质反过来将被肽质量指纹识别，这是一种新颖的、最先进的技术。拟议中的工作是一个试点项目，将寻找在不同温度下进化的模式细菌物种大肠杆菌种群中的薄弱环节，并测试低温种群中的薄弱环节是否在高温种群中得到加强。拟议的工作将为一种方法奠定基础，通过这种方法，可以确定任何生物体的基因组特征，以及与任何自然或人为压力有关的薄弱环节。潜在地，这些薄弱环节决定了生物体的生态和进化极限、它们能达到的最大年龄、对疾病的易感性等，但迄今为止还不可能确定薄弱环节本身。该项目也将是第一个证明肽质量指纹识别技术在这一科学领域问题上的适用性的项目。