New Transmission Electron Microscope for a Core EM Facility

该奖项为购买配备冷冻转移保持器、旋转保持器和CCD相机的透射电子显微镜提供支持。 该系统将取代位于生物系电子显微镜设施的20年前的仪器。该设施目前拥有扫描和透射电子显微镜以及先进的光学显微镜。 由EM设施主任、副主任和部门主席组成的咨询委员会监督仪器的操作和维护。新TEM的增强能力将使11个不同实验室的各种结构研究受益，包括对生活在高盐沃茨中的单细胞光合生物的研究;线粒体中蛋白质的定位和表征;收缩蛋白质功能的分析;鱼类进化适应的研究;与珊瑚有关的微生物的鉴定;以及DNA重组的分析。该仪器的使用应提供新的见解，不同的基本细胞生物学和进化问题，以及发挥重要作用，在少数民族服务机构的本科生的培训。 除了这些研究和培训角色，显微镜设施还参加了年度科学开放日，介绍了数百名社区成员从大圣地亚哥地区的学生和教师在该机构所做的研究。

This award provides support for purchase of a Transmission Electron Microscope equipped with cryotransfer holder, rotation holder, and CCD camera. The system will replace a 20 year old instrument located in the Electron Microscope Facility in the Department of Biology. The Facility presently houses both scanning and transmission electron microscopes as well as advanced light microscopes. An advisory committee, composed of the EM Facility Director, the Associate Director, and the Department Chair, supervises operation and maintenance of instruments. The enhanced capabilities of the new TEM will benefit diverse structural studies by 11 different laboratories, including studies of a unicellular photosynthetic organism that lives in hypersaline waters; localization and characterization of proteins in mitochondria; analysis of contractile protein function; study of evolutionary adaptations in fish; identification of microbes associated with corals; and analysis of DNA recombination. Use of the instrument should provide new insights into diverse basic cell biological and evolutionary questions, as well as playing an important role in the training of undergraduate students at a minority serving institution. In addition to these research and training roles, the microscopy facility also participates in an annual Sciences Open House that introduces hundreds of community members from the greater San Diego area to the research done by students and faculty at the institution.