DISSERTATION RESEARCH: Karyotypic Diversity and Species Radiation in the Genera Agrodiaetus, Lysandra and Plebicula (Lepidoptera: Lycaenidae)

论文研究：Agrodiaetus、Lysandra 和 Plebicula 属的核型多样性和物种辐射（鳞翅目：狼科）

• 批准号: 0309287
• 负责人: Naomi Pierce
• 金额: $ 1.15万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0309287&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Karyotypic; Diversity; Species

A grant has been awarded to Dr. Naomi E. Pierce and Mr. Nikolai Kandul at Harvard University to study the role of chromosomal evolution in species radiation. The karyotype, or number and structure of chromosomes in the nucleus of the cell, typically does not vary much from species to species. Humans, for example, have 23, while our closest relative, the chimpanzees, has 24. In contrast to this general pattern, three groups of butterflies, the genera Agrodiaetus, Lysandra and Plebicula, possess the greatest karyotype diversity in the animal kingdom, with chromosome numbers ranging from 7 to 225 among closely related species. Preliminary studies show that this chromosome diversity evolved independently within each of these groups. Dr. Pierce and Mr. Kandul will use this remarkable phenomenon to answer the following questions: Did karyotype diversification in these genera cause the production of new species, or is it merely the bi-product of other factors promoting speciation? What mechanisms have promoted karyotype diversification within these genera? In the course of the proposed research: (1) the evolutionary history of these genera will be reconstructed using characters from their DNA; (2) karyotypes will be examined and mapped on the evolutionary tree; (3) the amount of DNA in a single cell will be measured for species with different karyotypes; and (4) factors driving karyotype diversification will be assessed.In terms of its broader impact, the results of this research will illuminate mechanisms underlying genome instability. This has practical applications in understanding medical conditions; many cancers are correlated with changes in karyotype. The proposed research will also enlarge our knowledge of mechanisms underlying speciation. We read every day about extinction, and the crisis confronting the natural world. But ecosystems are the product of two processes: speciation, which adds new species to the mix, and extinction, which eliminates them. If we are truly to grapple with today's conservation issues, we need to understand both sides of the equation. Finally, the research has already involved collaboration between U.S., Spanish, Australian, Turkish and Russian scientists, and will continue to foster international communication and understanding.

已向Naomi E博士提供赠款。皮尔斯和尼古拉·坎杜尔先生在哈佛大学研究染色体进化在物种辐射中的作用。 核型，或细胞核中染色体的数量和结构，通常在物种之间没有太大差异。 例如，人类有23个，而我们的近亲黑猩猩有24个。 与此相反，三组蝴蝶，属Agrodiaetus，Lysandra和Plebicula，拥有最大的核型多样性在动物王国中，染色体数目从7到225之间的密切相关的物种。 初步研究表明，这种染色体多样性在这些群体中独立进化。 皮尔斯博士和坎杜尔先生将利用这一非凡的现象来回答以下问题：这些属的核型多样化是否导致了新物种的产生，或者它仅仅是促进物种形成的其他因素的副产品？ 是什么机制促进了这些属的核型多样化？ 在拟开展的研究过程中，（1）利用DNA特征重建这些属的进化历史，（2）对核型进行分析并绘制进化树，（3）对不同核型的物种进行单细胞DNA含量的测定，（4）对不同核型的物种进行单细胞DNA含量的测定，（5）对不同核型的物种进行单细胞DNA含量的测定。（4）分析染色体组型多样性的驱动因素。从更广泛的影响角度来看，本研究的结果将阐明基因组不稳定性的潜在机制。 这在理解医学状况方面有实际应用;许多癌症与核型的变化有关。 拟议中的研究还将扩大我们对物种形成机制的了解。 我们每天都读到关于物种灭绝的文章，以及自然界面临的危机。 但是生态系统是两个过程的产物：物种形成，它将新物种加入到混合物中，灭绝，它将它们消灭。 如果我们真的要解决今天的保护问题，我们需要了解等式的两边。 最后，这项研究已经涉及美国，西班牙、澳大利亚、土耳其和俄罗斯科学家，并将继续促进国际交流和理解。