An Inquiry-based Cell Biology Laboratory in which Student Research Teams Investigate the Heat Schock Response Using a Cell Culture Model

生物科学(61)这个项目正在斯通希尔学院建立一个以探究为基础的本科生细胞生物学实验室，以配合所需的讲座课程。该实验室课程是以先前资助的两个CCLI项目(0088185,9952672)为蓝本的。通过团队项目进行研究性学习的主题改编自第一个项目(0088085)，而热休克反应的统一主题是我们实施了第二个项目(9952672)中关于绿色荧光蛋白的类似主题。两个模块中的第一个模块指导学生基本的细胞和分子生物学技术，而第二个模块允许学生通过合作研究团队调查实验条件对CHO细胞中热休克蛋白表达的影响来展示他们使用这些技术的能力。学生的成绩以实验报告的形式传达，以科学文章、海报和口头报告的形式在向学院社区开放的小型研讨会上发表，每个报告都经过同行和教职员工的审查。拟议项目的目标是(1)建立一个以开放式发现为基础的方法来研究科学问题的实验室；(2)发展和改进关键的专业技能：假设和方案的开发，使用适当控制的实验，收集、分析和解释数据，以及形成结论；(3)培养合作学习的氛围；以及(4)发展和提高学生的分析、口头和书面交流技能。拟议活动的智力优点：细胞生物学实验室向学生介绍基因组学和蛋白质组学的基础知识，这两个高科技和具有挑战性的领域将彻底改变我们对生命过程的理解。因此，生物化学和生物专业的本科生获得这些领域的研究技术及其应用的实践知识和实践经验是至关重要的。拟议活动产生的广泛影响：完成细胞生物学课程和实验室的石山本科生将展示更强的研究技能。被选中的学生将作为导师、问题解决者和榜样，为学业上有天赋的、经济上处于不利地位的高中生在石山进行全日制研究，这是ACS SEED(面向弱势群体的暑期教育体验)项目的一部分。

Biological Sciences (61) This project is establishing an inquiry-based undergraduate Cell Biology Laboratory at Stonehill College to accompany the required lecture course. The laboratory course is modeled on two previously funded CCLI projects (0088185, 9952672). The theme of inquiry-based learning through team projects is adapted from the first project (0088085), and the unifying theme of heat shock response is our implementation of a similar theme on green fluorescent protein from the second project (9952672). The first of two modules instructs students in fundamental cell and molecular biology techniques, while the second allows the students to demonstrate their competencies with the techniques by working in collaborative research teams to investigate the effects of an experimental condition on the expression of heat shock proteins in CHO cells. Student results are communicated in the form of lab reports written in the style of a scientific article, a poster, and an oral presentation at a mini-symposium open to the College community, and each is peer- and faculty-reviewed. The objectives of the proposed project are (1) to establish a laboratory with an open-ended discovery-based approach to investigate a scientific question; (2) to develop and improve crucial professional skills: hypotheses and protocol development, experimentation using proper controls, collection, analysis, and interpretation of data, and formation of conclusions; (3) to foster an atmosphere of cooperative learning; and (4) to develop and improve students' analytical and oral and written communication skills.Intellectual Merit of the Proposed Activity: The Cell Biology Laboratory introduces students to the basics of genomics and proteonomics, two highly technical and challenging fields that will revolutionize our understanding of life processes. Therefore, it is essential that undergraduate Biochemistry and Biology majors obtain practical knowledge and hands-on experiences with the research techniques of these fields and their applications.Broader Impacts Resulting from the Proposed Activity: Stonehill undergraduates who complete the Cell Biology course and lab will demonstrate strengthened research skills. Selected students will serve as mentors, problem-solvers, and role models for academically-gifted, economically-disadvantaged high school students conducting fulltime research at Stonehill as part of the ACS's Project SEED (Summer Educational Experience for the Disadvantaged).