Incorporation of a Proteomics Laboratory Course Into the Undergraduate Biology Curriculum

纽约州立大学宾厄姆顿分校的生物系寻求加强其基因组学和蛋白质组学的本科生物学课程，该课程目前包括一门基于计算机的基因组学和蛋白质组学问题解决课程。我们正在改编瓦萨学院的蛋白质组学课程。通过从amersham购买蛋白质组学设备，包括Ettan MALDI-Pro质谱仪，我们正在建立一门动手的、跨学科的、以探究为基础的蛋白质组学实验室课程。学生们正在学习蛋白质组学的标准工具：用于蛋白质分离的二维电泳法和用于通过肽质量指纹图谱和肽序列鉴定蛋白质的MALDI-TOF质谱仪(MS)。学生们在逐步进行更复杂的练习的同时，也在学习这些技巧。在此之后，在生物、化学、心理学和生物工程系教员的指导下，正在进行一项调查研究项目。这些教师导师正在通过提供研究指导、样本准备辅导、指定阅读材料和财政支持来加强课程导师的努力，以支付供应成本。学生承诺在教师导师的实验室额外进行一学期的独立研究，以确保他们完成高质量的研究项目，并熟练使用蛋白质组学工具。政府强烈鼓励那些在科学界代表性不足的群体的学生选修这门课程。此外，质谱仪的使用还被纳入了附近大学教职员工和学生的暑期工作坊，以及其他8门生物、化学和生物工程课程，每年招收771名学生。我们正在通过网站、教育期刊以及在ASM和ASPB专业协会会议上的演讲来传播成果。

The Biology Department at the State University of New York at Binghamton seeks to enhance its undergraduate biology program in genomics and proteomics, which currently consists of a computer-based problem-solving course in Genomics and Proteomics. We are adapting the proteomics course from Vassar College. With acquisition of proteomics equipment including an Ettan MALDI-Pro mass spectrometer from Amersham, we are establishing a hands-on, interdisciplinary, inquiry-based Proteomics Laboratory course. Students are learning standard tools of proteomics: 2-D electrophoresis for protein separation and MALDI-ToF mass spectrometry (MS) for protein identification by peptide mass fingerprinting and peptide sequencing. Students are learning the techniques as they carry out incrementally more complex exercises. This is being followed by an investigative research project under the mentorship of faculty members from the biology, chemistry, psychology and bioengineering departments. These faculty mentors are enhancing the course instructors' efforts by providing research direction, tutelage in sample preparation, assigned readings, and financial support to cover supply costs. Students commit to an additional semester of independent research in the faculty mentors' laboratory to ensure that they complete a high-quality research project and develop proficiency with proteomics tools. Students belonging to groups underrepresented in science are being strongly encouraged to take the course. In addition, use of the mass spectrometer is being incorporated in summer workshops for faculty and students from nearby colleges as well as in 8 other biology, chemistry, and bioengineering courses, with annual enrollments of 771 students. We are disseminating results through a website, educational journals, and presentations at the ASM and ASPB professional society meetings.