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Cold Spring Harbor Laboratory Course on Proteomics

冷泉港蛋白质组学实验室课程

基本信息

批准号：
10395570
负责人：
DAVID J. STEWART
金额：
$ 12.33万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-03 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10395570
关键词：
Animal Model Animals Area Arts Bioinformatics Biological Biological Assay Biological Process Biology Characteristics Complex Coupled Course Content Data Data Analyses Development Dimensions Disease Ensure Equipment Exercise Faculty Fractionation Gel Genetic Genomics Goals Health High Pressure Liquid Chromatography Human Biology Individual Institutes Invertebrates Isotope Labeling Knowledge Label Laboratories Learning Mass Spectrum Analysis Measures Medical Methodology Methods Modeling Molecular Biology Monitor Mus Participant Peptides Physiological Post-Translational Protein Processing Postdoctoral Fellow Process Protein Analysis Proteins Proteome Proteomics Rana Rattus Regulation Research Research Personnel Sampling Scientist Sequence Analysis Series Shotguns Students System Systems Biology Talents Techniques Technology Training Update Zebrafish biological research design experience experimental study fly graduate student grasp high throughput technology human disease improved in vitro Model insight instructor laboratory curriculum laboratory experience lecturer lectures metabolomics peer programs protein complex protein expression protein profiling skills statistics stem cell technology student training tandem mass spectrometry work-study

项目摘要

Abstract CSHL Proteomics Course The proposed Cold Spring Harbor Laboratory Course on Proteomics is to be held each summer from 2018 through 2022. Proteomics is one of the pillar technologies of systems biology by which hundreds or thousands of proteins can be monitored and characterized simultaneously. In combination with genomics and metabolomics approaches, proteomics is an enabling core technology to probe biological activities in parallel, providing unprecedented analytical power into diverse biological processes in mammalian development and disease. Models of many human diseases have been developed in a wide variety of animal systems, in particular vertebrate models such as mouse, zebrafish, rat and frog, and invertebrate models such as worms and flies; and increasingly the use of new stem cell technologies is being harnessed to produce powerful in vitro models to be deployed alongside animal models. There is a growing need for implementation of systems biology approaches to these models, which necessitates an in-depth understanding of the challenges and pitfalls of various “high-throughput” technologies, achieved in part by hands-on and highly focused training. This intensive two week laboratory and lecture course will focus on cutting-edge proteomics approaches and technologies. Students will gain practical experience purifying and identifying protein complexes and enriching/characterizing post-translationally modified peptides using the latest methods. In the protein profiling portion of the course, students will gain hands-on experience in quantitative proteome analysis methods: they will be taught label-free and covalent isotopic-labeling approaches to differentially profile changes in proteomes. Students will be trained in high-sensitivity, microcapillary high pressure liquid chromatography (nanoHPLC) coupled with nanospray-ESI and tandem mass spectrometry. Both single dimension and multidimensional separation methods coupled to mass spectrometry will be taught. In the targeted proteomics section of the course, students will be taught to analyze and process shotgun proteomics data in order to accurately identify and quantify selected proteins: they will be trained to select and design transitions for targeted peptides and to setup and perform SRM/MRM mass spectrometry assays. They will learn to process and interpret the acquired data to measure and validate changing quantities of targeted proteins in a variety of biological samples. For all sections of the course, a strong emphasis will be placed on data analysis. Outside lecturers will discuss proteomics topics and methods not directly covered in the course including de novo sequence analysis; 2D-gel fractionations and detecting/quantifying protein-level changes using DIGE; advanced mass spectrometry methods; statistics; the analysis of intact proteins, and native mass spectrometry; and functional proteomics. The overall aim of the course is to provide each student with the fundamental knowledge and hands-on experience necessary to be able to perform proteomics experiments and properly analyze the resulting data. The long term goal is to train students to identify new opportunities and applications for proteomics approaches in their biological research and to learn how to integrate these into systems biology and model organism approaches to human biology and health.

摘要