2010 Project: Identification and Functional Analysis of Genes Required for Gametophyte Development

0313501SundaresanSundaresan这项拟议的研究将鉴定和确定拟南芥配子体基因的功能。将使用DS插入线进行配子体突变的突变筛选，从而鉴定出约400个配子体突变。被这些插入干扰的基因将通过序列进行鉴定，并发布在加州大学戴维斯分校的网站(http://www-plb.ucdavis.edu/Labs/sundar/).基因功能的鉴定将通过对突变体中的雌、雄配子体进行综合的遗传、细胞和发育研究来进行。特化的突变体将通过存放在ABRC库存中心公开提供。此外，我们将使用表达谱来识别雌配子体特有的基因。人们对开花植物配子体发育所需的基因和功能知之甚少。2010年项目的目标是确定所有拟南芥基因的功能，这将要求鉴定和鉴定配子体基因，配子体基因在植物繁殖中发挥关键作用。这些研究还将产生潜在的应用，以控制作物的繁殖。更广泛的影响：该项目将为两个参与机构的博士后研究员、研究生和本科生提供植物生物学研究方面的综合培训。将努力从任职人数不足的群体中招募个人参加这一项目。在本科生的情况下，私人投资机构将与其机构旨在鼓励少数族裔参与研究的特定计划合作。此外，该项目的参与者将通过与高中生的联系和通过中学的讲习班单元参与到学校的外联工作中。

0313501SundaresanThe proposed research will identify and determine functions of gametophytic genes in Arabidopsis. A mutant screen for gametophytic mutations using Ds insertion lines will be performed, resulting in the identification of an estimated 400 gametophytic mutants. The genes disrupted by these insertions will be identified by sequence and posted on a website at UC Davis (http://www-plb.ucdavis.edu/Labs/sundar/). Characterization of gene function will be carried out through integrated genetic, cellular and developmental study of the female and male gametophytes in the mutants. The characterized mutants will be made publicly available through deposition in the ABRC stock center. In addition, we will use expression profiling to identify female gametophyte-specific genes. Relatively little is known about the genes and functions required for gametophytic development in flowering plants. The aim of the 2010 project to determine the functions of all Arabidopsis genes will require the identification and characterization of gametophytic genes, which play a critical role in plant reproduction. These studies will also yield potential applications towards the control of reproduction in crop plants. Broader Impact: The project will provide comprehensive training in plant biology research for postdoctoral fellows, graduate students and undergraduate students at both participating institutions. Efforts will be made to recruit individuals from underrepresented groups to participate in this project. In the case of undergraduate students, the PIs will work with specific programs at their institutions intended for the purpose of encouraging minority participation in research. In addition, participants in the project will be involved in outreach efforts to schools through the attachment of high school students and through workshop modules in middle schools.