Laboratory Evolution of Biocatalysts for Methane Hydroxylation and Alkene Epoxidation

甲烷羟基化和烯烃环氧化生物催化剂的实验室进展

• 批准号: 0313567
• 负责人: Frances Arnold
• 金额: $ 45万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0313567&HistoricalAwards=false
• 关键词: Laboratory; Evolution; Biocatalysts; Methane; Hydroxylation

Hydroxylation and epoxidation reactions on hydrocarbons produce valuable intermediates for the chemicals and pharmaceuticals industries, but traditional oxidation processes are energy-or resource-intensive and produce unwanted and often environmentally hazardous side products. The objective of this research is to use a highly efficient alkane hydroxylase as the starting point for evolving catalysts for (1) the conversion of methane to methanol and (2) alkene epoxidation. Both of these goals are within reach, due to the special properties of the evolved alkane hydroxylase, which, as a side product of the previous evolution, acquired sufficient activity on small alkanes and on alkenes for screening mutant libraries for further improved catalysts. The biocatalysts discovered can be used in developing practical and clean hydroxylation and epoxidation processes.

烃的羟基化和环氧化反应产生用于化学品和制药工业的有价值的中间体，但传统的氧化工艺是能源或资源密集型的，并且产生不需要的并且通常对环境有害的副产物。本研究的目的是使用一种高效的烷烃羟化酶作为开发催化剂的起点，用于（1）甲烷转化为甲醇和（2）烯烃环氧化。 这两个目标都是可以实现的，由于演化的烷烃羟化酶的特殊性质，其作为先前演化的副产物，获得了对小烷烃和烯烃的足够活性，用于筛选突变体库以进一步改进催化剂。 所发现的生物催化剂可用于开发实用和清洁的羟基化和环氧化工艺。