A Microfabricated Cell Sorter for Molecular Evolution

用于分子进化的微加工细胞分选器

• 批准号: 9901495
• 负责人: Frances Arnold
• 金额: $ 10.91万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9901495&HistoricalAwards=false
• 关键词: Microfabricated; Cell; Sorter; Molecular; Evolution

The overall goal of this SGER Proposal is to determine if it is feasible to construct an economical, microfabricated cell sorter for sorting large numbers of bacterial populations generated in "directed evolotion" experiments. An appropriate elastomeric material will be identified that minimizes the adherence of bacterial cells to the sorter surface. A sorting method (identifies and separates tagged cells) will be identified and the appropriate software developed. The viability of the bacterial cells in the apparatus will be determined. This normally major effort is facilitated by the head-start the Quake Laboratory (Applied Physics, Caltech) has with a similar design for sizing and sorting single DNA molecules based on their fluorescent properties.

这项SGER提案的总体目标是确定是否可以构建一种经济的、微型制造的细胞分类器，用于对“定向进化”实验中产生的大量细菌种群进行分类。将确定一种适当的弹性材料，以最大限度地减少细菌细胞对分选机表面的粘附性。将确定一种分类方法(识别和分离标记的单元格)并开发适当的软件。仪器中细菌细胞的活性将被确定。这项通常主要的工作是由Quake实验室(应用物理，加州理工大学)率先进行的，该实验室具有类似的设计，可以根据单个DNA分子的荧光特性来确定其大小并进行分类。