Collaborative Research: The Environmental Sample Processor (ESP): A Device for Detecting Microorganisms In Situ Using Molecular Probe Technology

合作研究：环境样品处理器（ESP）：一种利用分子探针技术原位检测微生物的装置

• 批准号: 0314222
• 负责人: Christopher Scholin
• 金额: $ 131.92万
• 依托单位: Monterey Bay Aquarium Research Institute
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0314222&HistoricalAwards=false
• 关键词: Collaborative; Research; Environmental; Sample; Processor

This award provides funds to enhance the on-going Environmental Sample Processor (ESP) instrument development program. The (ESP) is a novel instrument that collects discrete water samples autonomously, concentrates microorganisms and automates application of DNA (or other) molecular probes to enable identification and quantification of particular species captured in situ, subsurface. The instrument transmits results of DNA probe array based assays in real- time to a shore based location for processing, interpretation and dissemination. In addition, the ESP archives discrete samples for nucleic acid, microscopic and toxin analyses for verifying real- time data from the probe arrays as well as facilitating discovery based analyses in the laboratory. In this three year project, the PI's propose to: 1) construct a second generation, internet- accessible ESP (2G ESP); 2) refine DNA probe arrays used in the ESP for detecting specific species; 3) develop toxin detection techniques that are applicable for use on the ESP in real-time; and 4) conduct field tests of the 2G ESP in Monterey Bay, California. Identification of specific nuisance, harmful or toxic algal species that pose widespread economic concerns and/or are known to negatively impact the health of humans and wildlife is emphasized.

该奖项提供资金以加强正在进行的环境样品处理器 (ESP) 仪器开发计划。 (ESP) 是一种新型仪器，可自主收集离散水样、浓缩微生物并自动应用 DNA（或其他）分子探针，以识别和定量在地下原位捕获的特定物种。该仪器将基于 DNA 探针阵列的检测结果实时传输到岸上位置进行处理、解释和传播。此外，ESP 还保存用于核酸、显微镜和毒素分析的离散样本，以验证探针阵列的实时数据并促进实验室中基于发现的分析。在这个为期三年的项目中，PI 建议： 1) 建设第二代可通过互联网访问的 ESP (2G ESP)； 2）完善ESP中用于检测特定物种的DNA探针阵列； 3）开发适用于ESP实时的毒素检测技术； 4) 在加利福尼亚州蒙特利湾对 2G ESP 进行现场测试。强调识别造成广泛经济问题和/或已知对人类和野生动物健康产生负面影响的特定滋扰、有害或有毒藻类物种。