Evaluation of DNA Probe-based Assays for Rapid Detection of Toxigenic Marine Diatoms

蒙特雷湾水族馆研究所的Christopher a . Scholin和新西兰纳尔逊考索恩研究所的Lesley L. Rhodes的这项合作研究项目的目标是：(1)确定采用大亚基核糖体RNA （LSU rRNA）靶向探针的试纸法是否可用于鉴定和定量与domoic酸（DA）生产相关的伪nitzschia产毒物种；一种与人类和野生动物疾病（失忆性贝类中毒）和经济损失有关的神经毒性物质，(2)将试纸试验的性能与基于显微镜的全细胞荧光试验进行比较，以及(3)评估该方法对作为新西兰贝类生物毒素监测计划一部分收集的样本进行常规分析的效用。该结果将作为一个模型，用于如何使用DNA探针近实时地检测其他水生有毒微生物。

9602576 Scholin This objectives of this collaborative research project between Christopher A. Scholin of the Monterey Bay Aquarium Research Institute and Lesley L. Rhodes of the Cawthorn Institute in Nelson, New Zealand are (1) to determine whether the dipstick assay incorporating large-subunit ribosomal RNA (LSU rRNA)- targeted probes is a viable method for identifying and quantifying toxigenic species of Pseudo-nitzschia associated with the production of domoic acid (DA), a neurotoxic substance linked to illness of humans and wildlife (amnesic shellfish poisoning) and to economic losses, (2) to compare the performance of the dipstick assay to a microscopy-based whole cell fluorescence assay, and (3) to assess the utility of the method for routine analyses of samples collected as part of the New Zealand shellfish biotoxin monitoring program. The results will serve as a model for how one might detect other aquatic-borne toxic micro organisms in near real-time using DNA probes.