Evaluation of DNA Probe-based Assays for Rapid Detection of Toxigenic Marine Diatoms

基于 DNA 探针的快速检测产毒海洋硅藻的评估

• 批准号: 9602576
• 负责人: Christopher Scholin
• 金额: $ 2.73万
• 依托单位: Monterey Bay Aquarium Research Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602576&HistoricalAwards=false
• 关键词: Evaluation; DNA; Probe; based; Assays

9602576 Scholin This objectives of this collaborative research project between Christopher A. Scholin of the Monterey Bay Aquarium Research Institute and Lesley L. Rhodes of the Cawthorn Institute in Nelson, New Zealand are (1) to determine whether the dipstick assay incorporating large-subunit ribosomal RNA (LSU rRNA)- targeted probes is a viable method for identifying and quantifying toxigenic species of Pseudo-nitzschia associated with the production of domoic acid (DA), a neurotoxic substance linked to illness of humans and wildlife (amnesic shellfish poisoning) and to economic losses, (2) to compare the performance of the dipstick assay to a microscopy-based whole cell fluorescence assay, and (3) to assess the utility of the method for routine analyses of samples collected as part of the New Zealand shellfish biotoxin monitoring program. The results will serve as a model for how one might detect other aquatic-borne toxic micro organisms in near real-time using DNA probes.

小行星9602576 这个合作研究项目的目的是克里斯托弗A。蒙特雷湾水族馆研究所的Scholin和LesleyL.新西兰纳尔逊的Cawthorn研究所的罗兹（Rhodes）等人（1）确定掺入大亚基核糖体RNA的试纸条测定是否（LSU rRNA）-靶向探针是鉴定和定量与软骨藻酸（DA）产生相关的假菱形藻属的产藻种的可行方法，一种与人类和野生动物疾病有关的神经毒性物质（失忆性贝类中毒）和经济损失，（2）为了比较试纸条测定法与基于显微镜的全细胞荧光测定法的性能，及（3）评估该方法对新西兰贝类生物毒素监测计划所收集样本的常规分析的效用。这些结果将作为一个模型，用于如何使用DNA探针近实时检测其他水生有毒微生物。