Acquisition of TEM Double Tilt Cooling Stage including Ultramicrotome for Specimen Preparation

采购 TEM 双倾斜冷却台，包括用于样品制备的超薄切片机

• 批准号: 0315579
• 负责人: Heike Gabrisch
• 金额: $ 9.5万
• 依托单位: University of New Orleans
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0315579&HistoricalAwards=false
• 关键词: Acquisition; TEM; Double; Tilt; Cooling

This grant supports the tandem of a cryo ultramicrotome and a double tilt cooling holder used in a study of phase transformations in LiCoO2, an electrode material in Li-ion batteries. The strain acting in LiCoO2 particles between the surface and the particle center is studied by CBED. This technique allows one to obtain information on crystal symmetry and dimensions of the unit cell from small regions. The results of systematic studies of lattice strains and lattice symmetry aid in understanding the driving forces and mechanisms of observed lattice transformation. Transmission electron microscopy (TEM) specimens are prepared in cross section by ultramicrotome. The research takes the approach of investigating battery materials by TEM to the next level, crystallographic details within single powder grains. While the application of TEM techniques to battery materials is fairly recent, it has proven successful in the discovery of the transformation from hexagonal to spinel phase that was not accessible by conventional diffraction techniques (x-ray and neutron diffraction). The instruments are of use in the development of new magnetic biological tracers for virus detection. The goal of this research is to bind gold-coated Fe and Fe3O4 nanoparticles to various antibodies as biological tracers for MRI detection. This research necessitates experiments to determine whether the tracer antibodies are capable of penetrating cell membranes and capillary walls. Tissue samples must be sectioned with a cryo-microtome. Another project involves the coating of metallic nanoparticles with polymers. This is studied in the TEM at liquid nitrogen temperatures to prevent beam damage of the coating layer. The results of these activities benefit society over a broad range: batteries are a substantial part of a technologically driven society. They operate lap top computers, cell phones and pacemakers. Batteries for medical applications need to be reliable and long lasting. In the immediate surrounding at UNO the new techniques will enhance the spectrum of possible investigations by TEM. AMRI is a joint project between the department of physics and chemistry. In many cases a crossing over to biological and medical applications and materials occurs that requires new investigation techniques. The facilities at AMRI are open to all scientists and students interested in using them. The local student population profits by working with state-of-the-art equipment on materials that stand at the forefront of materials development. Sttudents obtain an education that makes them highly qualified specialists. UNO is a university with a substantial minority student body.

该资助支持低温超薄切片机和双倾斜冷却保持器的串联，用于锂离子电池电极材料LiCoO2的相变研究。用CBED研究了LiCoO2颗粒表面和颗粒中心之间的应变。这种技术允许人们从小区域获得关于晶体对称性和晶胞尺寸的信息。晶格应变和晶格对称性的系统研究的结果有助于理解观察到的晶格转变的驱动力和机制。透射电子显微镜（TEM）标本制备的横截面超薄切片机。 该研究将TEM研究电池材料的方法提升到了一个新的水平，即单个粉末颗粒内的晶体学细节。虽然TEM技术在电池材料中的应用是相当新的，但它已被证明成功地发现了从六方相到尖晶石相的转变，这是常规衍射技术（X射线和中子衍射）无法实现的。 该仪器可用于开发用于病毒检测的新型磁性生物示踪剂。本研究的目的是将金包被的Fe和Fe3O4纳米颗粒结合到各种抗体上作为MRI检测的生物示踪剂。这项研究需要实验来确定示踪抗体是否能够穿透细胞膜和毛细血管壁。组织样本必须用冷冻切片机切片。另一个项目涉及用聚合物涂覆金属纳米颗粒。这是在液氮温度下的TEM中研究的，以防止涂层的束损伤。 这些活动的结果使社会受益广泛：电池是技术驱动社会的重要组成部分。他们操作笔记本电脑，手机和心脏起搏器。用于医疗应用的电池需要可靠和持久。在UNO的周边环境中，新技术将增强TEM可能的调查范围。AMRI是物理系和化学系之间的联合项目。在许多情况下，生物和医学应用和材料的交叉需要新的调查技术。AMRI的设施向所有有兴趣使用它们的科学家和学生开放。 当地的学生通过使用最先进的设备在材料开发的最前沿工作而受益。学生接受教育，使他们成为高素质的专家。UNO是一所拥有大量少数民族学生的大学。