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Regulation of Division and Elongation During Root Growth

根生长过程中分裂和伸长的调节

基本信息

批准号：
0316876
负责人：
Tobias Baskin
金额：
$ 44.5万
依托单位：
University of Massachusetts Amherst
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2003
资助国家：
美国
起止时间：
2003-09-01 至 2007-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0316876&HistoricalAwards=false
关键词：
Regulation Division Elongation During Root

项目摘要

Cell division and expansion are fundamental processes, however the relationship between them has been little studied. In a developing organ, it is not understood how the zones of division and rapid expansion are delimited; nor is it understood how rates of elongation and division are coordinated. Based on preliminary data, the PI hypothesizes first, that division ends well before rapid expansion begins; second, that the abrupt onset of rapid expansion is caused by a synchronous up-regulation of proton efflux and hydraulic conductivity of cell membranes; and third, that division and expansion are regulated reciprocally, so that a change in one elicits a proportional change in the other. The specific aims of the proposal are to test these hypotheses. To do so, The PI will use the plant root because of its linear organization, indeterminate growth, and experimental accessibility. The PI developed a kinematic method to quantify the spatial profiles of velocity, relative elongation rate and cell division rate. Profiles of velocity, relative elongation rate, cell division rate, and fluxes of selected ions will be quantified for conditions and genotypes that affect root growth, including development and water deficit. The PI will then perturb elongation and division and will quantify how the measured parameters change. Supporting or refuting the hypotheses will increase our understanding of fundamental growth processes in higher plants.

细胞的分裂和扩张是细胞的基本过程，但它们之间的关系却很少被研究。在一个发育中的器官中，分裂和快速扩张的区域是如何划定的，也不知道伸长和分裂的速度是如何协调的。基于初步数据，PI假设第一，分裂结束之前迅速扩大开始;第二，迅速扩大的突然发作是由质子流出和细胞膜的水力传导率的同步上调引起的;第三，分裂和扩张是相互调节的，因此一个的变化会导致另一个的成比例变化。该提案的具体目的是检验这些假设。为此，PI将使用植物根，因为它的线性组织，不确定的生长和实验可及性。PI开发了一种运动学方法来量化速度、相对伸长率和细胞分裂率的空间分布。将对影响根生长的条件和基因型（包括发育和水分亏缺）的速度、相对伸长率、细胞分裂率和选定离子通量的曲线进行量化。然后PI将扰动伸长和分裂，并将量化测量参数如何变化。支持或反驳这些假说将增加我们对高等植物基本生长过程的理解。