SBIR Phase I: Real-Time Micro-Array Imaging for Single Nucleotide Polymorphisms (SNPs) Detection

SBIR 第一阶段：用于单核苷酸多态性 (SNP) 检测的实时微阵列成像

• 批准号: 0318902
• 负责人: Val Golovlev
• 金额: $ 9.97万
• 依托单位: Sci-Tec, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0318902&HistoricalAwards=false
• 关键词: SBIR; Phase; Real; Time; Micro

This Small Business Innovation Research (SBIR) Phase I project is to establish the feasibility of a novel micro-array technology based on measurements of kinetics of hybridization of biopolymer molecules. By measuring kinetics of hybridization in real time, the perfectly and non-perfectly homologous DNA can be distinguished with much higher accuracy than by using conventional micro-arrays. An important innovative component of this technology is the labeling of target DNA by colloidal gold particles that are of the order of hundreds of nanometers in diameter. This labeling technique significantly increases the detection sensitivity and can be implemented using a very inexpensive detection system. Prior work has shown that the use of gold particles can increase the speed of hybridization to allow complete analysis in minutes, whereas conventional micro-array protocols require many hours. The proposed system is advantageous for many micro-array applications, including screening of single nucleotide polymorphisms (SNPs), when high hybridization specificity is required, and performing express micro-array analysis during time-critical medical procedures such as surgery.The commercial applications of this project include drug screening, nucleic acid identification and sequencing, single base mutation screening, and gene expression analysis. The increasing applications of micro-arrays in medical research will create additional opportunities for introducing the proposed technology into routine clinical practice.

这个小型企业创新研究 (SBIR) 第一阶段项目旨在建立基于生物聚合物分子杂交动力学测量的新型微阵列技术的可行性。 通过实时测量杂交动力学，可以比使用传统微阵列以更高的精度区分完全和非完全同源DNA。 该技术的一个重要创新部分是通过直径数百纳米的胶体金颗粒来标记目标 DNA。 这种标记技术显着提高了检测灵敏度，并且可以使用非常便宜的检测系统来实现。 先前的研究表明，使用金颗粒可以提高杂交速度，从而可以在几分钟内完成分析，而传统的微阵列方案则需要数小时。 该系统有利于许多微阵列应用，包括需要高杂交特异性时的单核苷酸多态性（SNP）筛选，以及在手术等时间关键的医疗过程中进行表达微阵列分析。该项目的商业应用包括药物筛选、核酸鉴定和测序、单碱基突变筛选和基因表达分析。 微阵列在医学研究中的日益增长的应用将为将所提出的技术引入常规临床实践创造更多机会。