SBIR Phase I: Biosensor Signal Amplification by ElectroWetting On Dielectric (EWOD) Droplet Transport and Distillation

SBIR 第一阶段：通过电介质电润湿 (EWOD) 液滴传输和蒸馏来放大生物传感器信号

• 批准号: 0320082
• 负责人: Wayne Liu
• 金额: $ 10万
• 依托单位: Core MicroSolutions, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0320082&HistoricalAwards=false
• 关键词: SBIR; Phase; Biosensor; Signal; Amplification

This Small Business Innovation Research (SBIR) Phase I project seeks to apply electrowetting on dielectric (EWOD) microdroplet transport technology and natural evaporation effects to increase biosensor sample concentration levels via droplet distillation. EWOD technology can generate droplets from an on-chip reservoir and achieve pad-by-pad transport of droplets over an electrode array. EWOD capabilities can digitize a fluidic volume into droplets for serial transport and distillation over a sensing site and provide high-speed droplet transport to "wash" a sensor free of unhybridized markers to reduce false positives. This proposed research will apply image recognition software and conductive sensing to evaluate the buildup of fluorescent microspheres and salinity over a sensing site achieved by droplet distillation. It is anticipated that EWOD capabilities will improve commercial biosensor performance by concentrating target molecules and reducing false positives with high-speed washing. The commercial applications of this project will be in the broad field of biosensing. The product will be utilized wherever low concentration measurements and multiple sampling are needed.

该小型企业创新研究（SBIR）第一阶段项目旨在应用电介质（EWOD）微滴传输技术和自然蒸发效应，通过液滴蒸馏提高生物传感器样品浓度水平。 EWOD技术可以从芯片上的储液器产生液滴，并实现液滴在电极阵列上的逐焊盘传输。 EWOD能力可以将流体体积混合成液滴，用于在感测位点上连续运输和蒸馏，并提供高速液滴运输以“洗涤”传感器使其不含未杂交的标记物，以减少假阳性。 这项拟议的研究将应用图像识别软件和传导传感来评估通过液滴蒸馏实现的传感点上的荧光微球和盐度的积累。 预计EWOD能力将通过浓缩目标分子和减少高速洗涤的假阳性来提高商业生物传感器的性能。该项目的商业应用将在生物传感的广泛领域。 该产品将用于任何需要低浓度测量和多次采样的地方。