STTR Phase I: Analysis of Comprehensive Two Dimensional Gas Chromatography with Mass Spectrometry for High-Throughput Metabolomics

STTR 第一阶段：高通量代谢组学综合二维气相色谱与质谱分析

• 批准号: 0320427
• 负责人: Erik Nilsson
• 金额: $ 10万
• 依托单位: INSILICOS, LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0320427&HistoricalAwards=false
• 关键词: STTR; Phase; Analysis; Comprehensive; Two

This Small Business Technology Transfer (STTR) Phase I project will investigate a new method for analyzing metabolites using novel instrumentation and software. Currently, metabolomics offers critical new information to pharmaceutical and other biological research. However, few researchers can exploit metabolomics, because appropriate methods have not been developed. Recently, gas chromatography has been successfully applied to metabolomics, although peak capacities have been insufficient. This project will combine a leading multidimensional gas chromatography research facility with a seasoned team of software experts, to bring the power of multidimensional GC to metabolomics. Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry detection (GC x GC xTOF-MS) will be combined with pattern recognition to analyze the reaction of metabolites of methylotrophic bacteria to perturbations. The collaborative effort between the Synovec Laboratory at the University of Washington and the Company will produce a software platform for analysis of GC x GC x TOF-MS data. This platform will use data management and analysis technologies that have not previously been commercially available for GC x GC x TOF-MS, and enable GC x GC x TOF-MS for high throughput metabolite analysis. The result will be an enabling technology for the elucidation of biological function.The commercial applications of this project include metabolomics and proteomics. The current market for proteomics is over $1 billion per year worldwide and growing at the rate of 34% per year. The market for metabolomics is $38 million, and growing at the rate of 46% per year. This project will address the current unmet need for multidimensional separations software in both metabolomics and proteomics.

这个小企业技术转让（STTR）第一阶段项目将研究一种使用新型仪器和软件分析代谢物的新方法。目前，代谢组学为药物和其他生物学研究提供了重要的新信息。然而，很少有研究人员可以利用代谢组学，因为还没有开发出合适的方法。最近，气相色谱法已成功地应用于代谢组学，虽然峰容量一直不足。该项目将联合收割机与经验丰富的软件专家团队结合，将多维GC的力量带到代谢组学中。全面的二维气相色谱-飞行时间质谱检测（GC x GC xTOF-MS）将与模式识别相结合，分析甲基营养菌代谢产物对扰动的反应。华盛顿大学的Synovec实验室和公司之间的合作将产生一个用于分析GC x GC x TOF-MS数据的软件平台。该平台将使用GC x GC x TOF-MS之前尚未商业化的数据管理和分析技术，并使GC x GC x TOF-MS能够进行高通量代谢物分析。该项目的商业应用包括代谢组学和蛋白质组学。目前全球蛋白质组学市场每年超过10亿美元，并以每年34%的速度增长。代谢组学的市场规模为3800万美元，并以每年46%的速度增长。该项目将解决目前代谢组学和蛋白质组学中对多维分离软件的未满足需求。