SBIR Phase II: A Surface Acoustic Wave Based Ion Source

SBIR 第二阶段：基于声表面波的离子源

• 批准号: 1330459
• 负责人: Erik Nilsson
• 金额: $ 45.23万
• 依托单位: Deurion LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-10-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330459&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Surface; Acoustic

This Small Business Innovation Research (SBIR) Phase II project will develop a high--?]throughput --?] surface acoustic wave nebulization (HTP--?]SAWN) ion source for mass spectrometry (MS). The SAWN ion source developed during phase I resulted in a device that has competitive performance between the two most popular ionization techniques for MS: electrospray ionization (ESI) and matrix--?]assisted laser desorption ionization (MALDI). SAWN offers the convenience of ionization from a planar surface like MALDI, but the performance characteristics of ESI at atmospheric pressure (Heron et al. Anal. Chem. 2010). However, at the end of phase I SAWN remains low throughput and at an early developmental stage technologically. The phase II proposal will refine SAWN performance by coupling it to a novel MS interface that efficiently samples solvated analytes produced by SAWN making it immediately available on all major manufacturer?fs instruments. Phase II will result in an 1) optimized piezoelectric wafer by investigating options other than lithium niobate; 2) automated, robotic, HTP--?]SAWN that will double the throughput of an existing ESI--?]based assay; and 3) a more economical to manufacture and efficient to operate concentric ring interdigitated transducer electrode design developed in phase I. The broader impact/commercial potential of this project will provide a high--?]throughput --?] surface acoustic wave nebulization (HTP--?]SAWN) ion source for mass spectrometry that offers ease of use; lower ion energetics and the capability to instantaneously monitor chemical reactions. SAWN has been shown by direct comparison to generate ions of lower energy (Huang et al. JASMS 2012; Yoon et al. Anal Chem 2012) than ESI and by inference MALDI. In addition to HTP--?]SAWN, phase II will result in SAWN being compatible with all major manufacturer?fs instruments. SAWN will also be combined with direct analysis in real time (DART) to expand the capability of SAWN and DART; e.g. the combination will provide secondary ionization of compounds in emulsions desorbed from the SAWN chip into the DART stream. Additionally, dried blood spot analysis by HTP--?]SAWN will be developed using a newborn error assay for lysosomal storage disorders as an example. This assay will be used to demonstrate the potential of SAWN for immediate analysis of reaction products. SAWN has the broader potential for direct analysis any number of chemical or biochemical reactions within seconds of them having been carried out from monitoring cellular secretions or bacterial communication.

这个小型企业创新研究 (SBIR) 第二阶段项目将开发一种用于质谱 (MS) 的高--?]通量--?]表面声波雾化 (HTP--?]SAWN) 离子源。在第一阶段开发的 SAWN 离子源产生的设备在两种最流行的 MS 电离技术之间具有竞争性的性能：电喷雾电离 (ESI) 和基质辅助激光解吸电离 (MALDI)。 SAWN 提供了从 MALDI 等平面电离的便利性，但提供了大气压下 ESI 的性能特征（Heron 等人 Anal. Chem. 2010）。然而，在第一阶段结束时，SAWN 的吞吐量仍然较低，并且在技术上仍处于早期开发阶段。第二阶段提案将通过将 SAWN 与新型 MS 接口耦合来改进 SAWN 性能，该接口可以有效地对 SAWN 产生的溶剂化分析物进行采样，使其立即在所有主要制造商的 fs 仪器上可用。第二阶段将通过研究铌酸锂以外的选项来产生 1) 优化的压电晶片； 2) 自动化、机器人、HTP--?]SAWN 将使现有基于 ESI--?] 的测定的通量增加一倍； 3) 在第一阶段开发的制造更经济、操作更高效的同心环叉指换能器电极设计。该项目更广泛的影响/商业潜力将为质谱分析提供高--?]吞吐量--?]表面声波雾化（HTP--?]SAWN）离子源，且易于使用；较低的离子能量和即时监测化学反应的能力。通过直接比较表明，SAWN 产生的离子能量低于 ESI（Huang 等人，JASMS 2012；Yoon 等人，Anal Chem 2012），并通过 MALDI 进行推断。除了 HTP--?]SAWN 之外，第二阶段将使 SAWN 与所有主要制造商的仪器兼容。 SAWN还将与实时直接分析（DART）相结合，扩展SAWN和DART的能力；例如该组合将为从 SAWN 芯片解吸到 DART 流中的乳液中的化合物提供二次电离。此外，将以溶酶体贮积症新生儿错误测定为例，开发通过 HTP--β]SAWN 进行的干血斑分析。该测定将用于证明 SAWN 对反应产物进行即时分析的潜力。 SAWN 具有更广泛的潜力，可以通过监测细胞分泌物或细菌通讯，在数秒内直接分析任何数量的化学或生化反应。