Analysis of a Newly-Identified Voltage-Activated Potassium Current in Drosophila

新发现的果蝇电压激活钾电流的分析

• 批准号: 0322461
• 负责人: Satpal Singh
• 金额: $ 39万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0322461&HistoricalAwards=false
• 关键词: Analysis; Newly; Identified; Voltage; Activated

Potassium channels are cellular proteins that play a vital role in physiological functioning of the nerves, muscles and other cells. A previously unknown novel type of potassium channel (named IKF) has been identified. This project will study: (i) how this channel functions, (ii) what pharmacological agents interact with this channel to either inhibit or enhance its activity, and (iii) what intracellular signaling mechanisms are involved in modulating this channel. These channel properties will be studied using methods that measure electrical activity of the channel in a model system that is well suited to genetic manipulation. The electrical measurements will provide information about the functioning of the channel. Effects of pharmacological agents will be studied by measuring the function of the channel after applying various drugs and toxins. Similarly, the use of drugs and genetic perturbations that effect the functioning of the channel will provide information on pathways that modulate the channel.The recent genomic revolution has led to the discovery of a large number of genes that code for potassium channels. In contrast, relatively few channels, including the IKF channel, have been identified at the physiological level. This project will help understand the physiological role of the IKF channel and will subsequently allow the establishment of a link between the channel and the gene that codes for it. The study will provide a fundamental framework for understanding the role of IKF and related channels in normal cellular physiology. Furthermore, this work will contribute to the infrastructure of science by providing training to undergraduate and graduate students pursing careers in the biological and the pharmacological sciences.

钾通道是细胞蛋白质，在神经、肌肉和其他细胞的生理功能中起着至关重要的作用。一种以前未知的新型钾通道（命名为IKF）已被确定。该项目将研究：（i）该通道如何起作用，（ii）什么药理学试剂与该通道相互作用以抑制或增强其活性，以及（iii）什么胞内信号传导机制参与调节该通道。这些通道特性将使用测量模型系统中通道的电活动的方法进行研究，该模型系统非常适合于遗传操作。电测量将提供有关通道功能的信息。通过测量应用各种药物和毒素后通道的功能来研究药理学试剂的作用。同样地，药物的使用和影响通道功能的遗传扰动将提供关于调节通道的途径的信息。最近的基因组革命导致发现了大量编码钾通道的基因。相比之下，相对较少的通道，包括IKF通道，已被确定在生理水平。 该项目将有助于理解IKF通道的生理作用，并随后允许建立通道和编码它的基因之间的联系。该研究将为理解IKF和相关通道在正常细胞生理学中的作用提供基本框架。 此外，这项工作将有助于科学的基础设施，提供培训，本科生和研究生追求的职业生涯在生物和药理科学。