Mutational Analysis of Potassium Currents in Drosophila

小行星9604457 这些研究旨在了解钾电流的功能和调节的分子机制，这对许多细胞功能都很重要。 果蝇幼虫肌肉有两个电压激活的钾电流和两个钙激活的钾电流。这些通道中的两个已经被研究了有选择性地破坏它们的突变。 该研究者在其他通道中鉴定了6种突变：破坏IK的Z66突变体和影响IA的P40。 该项目将克隆这些基因并进行测序。 这些信息将表明基因产物的性质，以及它们是通道的结构亚基还是通道的调节子。还将对P40突变体进行电生理学研究，以确认该基因的定位，并提供关于IA通道功能的信息。 这些研究将提供重要的信息，以帮助我们了解这些钾通道。 %%% K+通道在多种细胞过程中发挥关键作用。 果蝇拥有一套优秀的遗传工具，可用于研究许多生物现象，包括离子通道功能。 本研究的结果将有助于了解钾通道功能和调节的分子机制。 ***

9604457 Singh These studies are aimed at understanding the molecular mechanisms underlying the function and regulation of potassium currents, which are important for many cellular functions. Larval muscles of Drosophila have two voltage activated and two calcium activated potassium currents. Two of these channels have been investigated with mutations that selectively disrupt them. This investigator has identified 6 mutations in the other channels: the Z66 mutant which disrupts IK, and P40 which affects IA. This project will clone and sequence these genes. This information will indicate the nature of the gene products and whether they are structural subunits of the channels or regulators of them. Electrophysiological studies will also be done with the P40 mutant to confirm the mapping of this gene, and provide information on the functioning of IA channels. These studies will provide important information to aid our understanding of these potassium channels. %%% K+ channels play a key role in a wide variety of cellular processes. With an excellent repertoire of available genetic tools, Drosophila is useful in the study of many biological phenomena including ion channel function. The result of this research will aid in the understanding of the molecular mechanisms involved in the function and regulation of potassium channels. ***