The Influence of Cobalt and Cadmium Speciation on Marine Cyanobacteria in the Costa Rica Upwelling Dome and at Station Aloha

钴和镉形态对哥斯达黎加上升穹顶和阿罗哈站海洋蓝藻的影响

• 批准号: 0327225
• 负责人: Mak Saito
• 金额: $ 37.19万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0327225&HistoricalAwards=false
• 关键词: Influence; Cobalt; Cadmium; Speciation; Marine

ABSTRACTOCE-0327225While the influence of iron on phytoplankton growth has been the subject of extensive investigations, comparatively little work has been conducted on the influence of other trace metals such as cobalt, cadmium and zinc. Building on results from culture studies indicating differences in growth rates depending on trace metal concentrations, a scientist from Woods Hole Oceanographic Institution hypothesizes that shifts in relative abundances and speciation of trace elements could influence the composition of phytoplankton assemblages. To test the hypothesis, the investigator plans to examine the influence of cobalt and cadmium speciation on Synechococcus and Crocosphaera at two sites in the Pacific Ocean. In addition, he will determine the distribution of cobalt across transects in the Eastern Equatorial Pacific to improve our understanding of the global biogeochemical cycle of cobalt. To attain these goals, the scientist will develop and calibrate a cadmium speciation method, measure cobalt and cadmium speciation on vertical profiles, analyze cyanobacterial abundances relative to cadmium and cobalt speciation, conduct physiological experiments to determine the sensitivity of nitrogen fixing cyanobacteria to cobalt and zinc limitations and cadmium toxicity, analyze functional gene expression of metalloenzymes in Synechococcus and assess changes in cell number of Crocosphaera and nitrogen fixation gene expression.

ABSTRACTOCE-0327225 虽然铁对浮游植物生长的影响已成为广泛研究的主题，但对其他微量金属（如钴、镉和锌）的影响的研究相对较少。 伍兹霍尔海洋研究所的一位科学家根据培养研究的结果表明生长速率因微量金属浓度而存在差异，推测微量元素相对丰度和物种形成的变化可能会影响浮游植物组合的组成。 为了检验这一假设，研究人员计划在太平洋的两个地点研究钴和镉形态对聚球藻和鳄鱼球菌的影响。 此外，他还将确定东赤道太平洋各断面中钴的分布，以增进我们对全球钴生物地球化学循环的了解。 为了实现这些目标，科学家将开发和校准镉形态方法，测量垂直剖面上的钴和镉形态，分析蓝藻相对于镉和钴形态的丰度，进行生理实验以确定固氮蓝藻对钴和锌限制以及镉毒性的敏感性，分析蓝藻的功能基因表达 聚球藻中的金属酶并评估鳄鱼球菌细胞数量和固氮基因表达的变化。