Physiological, molecular and epigenetic approaches to investigate regulation of leaf senescence by chloroplast derived signals

大麦旗叶在叶片衰老过程中的分子和表观遗传变化将被描述在大麦旗叶中，这些旗叶是从田间采集的，并且已经被描述了各种光合作用参数。该项目的一个目标是阐明光合作用装置中与早期发育相关的事件，这些事件发生在细胞核中衰老相关基因的表达之前。另一个目标是确定叶绿体信号和逆行信号的可能媒介，这些信号影响细胞核中与衰老相关的染色质修饰和基因表达。基于这一领域的试验数据，将提出叶绿体起始信号调节衰老机制的假说。这些假设将在未来的项目中得到检验，这些项目将在WB、HK、KH和KK提供一揽子申请的跨学科背景下进行。

Molecular and epigenetic changes during leaf senescence will be characterized in barley flag leaves which have been collected from fields and have been characterized for various photosynthetic parameters. One objective of the project is to elucidate early development related events in the photosynthetic apparatus which precede the expression of senescence associated genes in the nucleus. Another objective is to identify chloroplast signals and putative mediators of retrograde signalling affecting senescence associated chromatin modifications and gene expression in the nucleus. Based on the data of this field trial hypotheses on the mechanism of senescence regulation by chloroplast originating signals will be developed. These hypotheses will be tested in future projects which will be conducted in an interdisciplinary context of a package application by WB, HK, KH and KK.