US-Germany Cooperative Research: Mechanistic Investigation of the Ribosomal Peptidyl Transferase Reaction

美德合作研究：核糖体肽基转移酶反应的机理研究

• 批准号: 0339595
• 负责人: Scott Strobel
• 金额: --
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0339595&HistoricalAwards=false
• 关键词: US; Germany; Cooperative; Research; Mechanistic

0339595StrobelThis award supports Scott A. Strobel and students from Yale University in a collaboration with Marina Rodnina of the Institute for Physical Biochemistry at the University of Witten/Herdecke, Germany. The focus of the collaborative research will be the catalytic mechanism of the ribosomal peptidyl transferase reaction. Recent structural results from ribosome crystallography have identified the location of the peptidyl transferase center and suggested which rRNA bases are most likely to be involved in catalysis. However, functional identification of catalytic groups by quantitative measurements is lacking for a series of challenging technical reasons. The collaboration between the German and US groups aims to solve these problems by combining the technical expertise of the two research groups. The mechanistic investigation will be undertaken by combining rapid kinetic methods (German group) with the affinity purification of native ribosomal mutants, and the use of new synthetic substrate analogues (US group). This award will also facilitate interaction and collaboration between the young scientists in the two research groups. The international experience will provide two graduate students and one postdoctoral associate with a broader perspective of science and culture. It will also help them to see their research projects in the expanded context of an international collaboration.

0339595StrobelThis award supports Scott A. Strobel和耶鲁大学的学生与德国维滕/埃尔德克大学物理生物化学研究所的Marina Rodnina合作。 合作研究的重点将是核糖体肽基转移酶反应的催化机制。 核糖体晶体学的最新结构结果已经确定了肽基转移酶中心的位置，并表明哪些rRNA碱基最有可能参与催化。 然而，由于一系列具有挑战性的技术原因，缺乏通过定量测量对催化基团进行功能鉴定。 德国和美国研究小组之间的合作旨在通过结合两个研究小组的技术专长来解决这些问题。 将通过将快速动力学方法（德国组）与天然核糖体突变体的亲和纯化以及使用新的合成底物类似物（美国组）相结合来进行机理研究。 该奖项还将促进两个研究小组的年轻科学家之间的互动和合作。 国际经验将为两名研究生和一名博士后提供更广阔的科学和文化视野。它还将帮助他们在国际合作的扩大背景下看待他们的研究项目。