Instrumentation for Time Correlated Single Photon Counting

在化学研究仪器和设施（CRIF）项目的支持下，马里兰大学化学系将获得用于时间相关单光子计数的仪器。该设备将加强许多领域的研究，包括：a)蛋白质诱导的DNA构象群的单分子研究；B)测量短寿命低量子产率物种的荧光寿命；C)复合荧光动力学和新型分子表面活性剂吸附于液/液界面的重定向研究；d)环境系统中自由基化学的研究。测量时间分辨荧光使研究人员能够直接了解探针周围的局部环境和动态。用于监测时间分辨荧光的技术已广泛应用于光生物学等已建立的领域以及材料化学等新兴领域。

With support from the Chemistry Research Instrumentation and Facilities (CRIF) Program, the Department of Chemistry at the University of Maryland in College Park will acquire instrumentation for time correlated single photon counting. This equipment will enhance research in a number of areas including the following: a) single-molecule studies of protein-induced DNA conformational populations; b) measurement of fluorescence lifetimes from short-lived low quantum yield species; c) studies of complex fluorescence kinetics and reorientation of new molecular surfactants adsorbed to liquid/liquid interfaces; and d) investigations of radical chemistry in environmental systems. Measuring time resolved fluorescence affords researchers direct insight into the local environment and dynamics around a probe. Techniques developed to monitor time resolved fluorescence have been broadly applied to established fields such as photobiology as well as newly emerging areas in materials chemistry.