Neurogenetic Analysis of Neuropeptide Receptor Function in Drosophila

果蝇神经肽受体功能的神经遗传学分析

• 批准号: 0343699
• 负责人: John Ewer
• 金额: $ 27.78万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-15 至 2007-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0343699&HistoricalAwards=false
• 关键词: Neurogenetic; Analysis; Neuropeptide; Receptor; Function

Neuropeptides are small signaling molecules that control the development, physiology, and behavior of animals. This proposal uses genetic approaches in the fruit fly, Drosophila melanogaster, to understand how neuropeptides regulate ecdysis, the behavior used by insects to shed their old exoskeleton (cuticle) at the end of each molt Ecdysis is controlled by a series of interacting neuropeptides. The stereotyped nature of the behavior, coupled with its complex regulation, has made it a premier system for understanding how neuropeptides cause concerted and developmentally appropriate changes in behavior and physiology. Our previous work has established that the neuropeptide Crustacean Cardioactive Peptide (CCAP) plays an important role in regulating Drosophila ecdysis, consistent with findings from the moth. It also revealed that the actions of CCAP are more complex than predicted. This proposal will uses the genetic tools available in Drosophila to understand the bases for CCAP's actions by determining the expression of the CCAP receptor (CCAP-R), and analyzing the role of the different CCAP-R-expressing cells in the control of ecdysis. This work will further our understanding of how neuropeptides control animal behavior and development. It will also specifically advance the understanding of the control of ecdysis behavior, a behavior that is essential for the survival of all insects. Thus, information gained from this research may lead to the development of useful agents of insect control. The research proposed here offers a unique opportunity to receive an unusually broad training in Drosophila biology, including neuroendocrinology, genetics, and behavior. In addition, the results of this research will be used in lecture and practical courses offered by the PI.

神经肽是控制动物发育、生理和行为的小信号分子。该提案使用遗传学方法在果蝇，果蝇，了解神经肽如何调节蜕皮，昆虫用于摆脱旧的外骨骼（角质层）在每次蜕皮结束时蜕皮的行为是由一系列相互作用的神经肽控制的。这种行为的定型性质，加上其复杂的调节，使其成为了解神经肽如何引起行为和生理学的协调和发育适当变化的首要系统。我们以前的工作已经确定，神经肽甲壳动物蜕皮活性肽（CCAP）在调节果蝇蜕皮中起着重要作用，与蛾的发现一致。研究还表明，CCAP的作用比预期的要复杂。该提案将使用果蝇中可用的遗传工具，通过确定CCAP受体（CCAP-R）的表达，并分析不同CCAP-R表达细胞在蜕皮控制中的作用，来了解CCAP作用的基础。这项工作将进一步加深我们对神经肽如何控制动物行为和发育的理解。它还将特别推进对蜕皮行为控制的理解，蜕皮行为对所有昆虫的生存至关重要。因此，从这项研究中获得的信息可能会导致有用的昆虫控制剂的发展。这里提出的研究提供了一个独特的机会，接受果蝇生物学，包括神经内分泌学，遗传学和行为学的异常广泛的培训。此外，本研究的结果将用于PI提供的讲座和实践课程。