RUI: Identification Of Drosophila Wing Shape Genes Using Microarrays And P-Elements

RUI：利用微阵列和 P 元件鉴定果蝇翅膀形状基因

• 批准号: 0344003
• 负责人: Kenneth Weber
• 金额: $ 36.68万
• 依托单位: University of Southern Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0344003&HistoricalAwards=false
• 关键词: RUI; Identification; Drosophila; Wing; Shape

This project will study how genes control the shapes of living things. It will identify specific genes that affect the shapes of fly wings, and will assess their relative effects and possible modes of action. Fly wings offer a fast, cost-effective way to investigate the basic principles of genetic shape control, because they can be easily projected and magnified, and their shapes can be rapidly measured with a computer. This makes it possible to analyze thousands of measurements of individual differences, which are required to study the small effects of many genes. Previous work in mice and flies indicates that many minor genes are involved in shape control. In this study the new technique of microarray analysis will be combined with massively replicated measurements to find and identify these genes. In many cases we know how genes guide cells to differentiate into the separate parts of organisms, but not much is known about how growth is subsequently regulated to determine the final shapes of parts. Understanding differential growth regulation in normal cells should advance our knowledge in areas of medical interest, with possible applications to abnormal growth, birth defects, and the origins of cancer. Frequently, basic research in fruit fly genetics has led to major advances in human genetics.

这个项目将研究基因如何控制生物的形状。它将识别影响苍蝇翅膀形状的特定基因，并评估它们的相对影响和可能的作用模式。苍蝇翅膀提供了一种快速、经济高效的方法来研究遗传形状控制的基本原理，因为它们可以很容易地投影和放大，而且它们的形状可以用计算机快速测量。这使得分析数千个个体差异的测量成为可能，这是研究许多基因的微小影响所必需的。之前在老鼠和苍蝇身上的研究表明，许多微小基因参与了形状控制。在这项研究中，微阵列分析的新技术将与大规模重复测量相结合，以发现和识别这些基因。在许多情况下，我们知道基因如何引导细胞分化为生物体的不同部分，但对于随后如何调节生长以决定部分的最终形状，我们知之甚少。了解正常细胞的不同生长调控应该会促进我们在医学领域的知识，并可能应用于异常生长、出生缺陷和癌症的起源。果蝇遗传学的基础研究经常导致人类遗传学的重大进步。