Are Plant Root-Mycobacterium Interactions Beneficial in Remediation of Polyaromatic Hydrocarbons?

植物根-分枝杆菌相互作用是否有益于多环芳烃的修复？

• 批准号: 0346539
• 负责人: Anne Anderson
• 金额: --
• 依托单位: Utah State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2009-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0346539&HistoricalAwards=false
• 关键词: Plant; Root; Mycobacterium; Interactions; Beneficial

PIs: AndersonAre plant root-mycobacterium interactions beneficial in remediation of polyaromatic hydrocarbons? PAH-contaminated soils pose environmental and health hazards. Phytoremediation is a cost effective method for on-site clean-up. It is well suited for large surface areas such as those designated as Brownfields within urban settings or sites where soil excavation and removal is difficult. This proposal focuses on understanding more of the ecology of the group of mycobacterium that has PAH-degrading potential. Currently there is little knowledge of how such mycobacteria interact with plant roots and whether this association has positive impacts on the metabolism of the plant and/or the microbe to promote bioremediation. The hypotheses to be tested are: 1) The presence of roots colonized by PAH-degrading mycobacteria improves the bioavailability of a model, recalcitrant PAH, pyrene.2) The mineralization of pyrene is enhanced by the interaction of the roots with the mycobacterium. 3) Colonization of the root requires discrete interactions between the mycobacterium and root surface.4) Colonization of the root permits the expression of the gene in the mycobacterium encoding the first enzyme involved in PAH degradation, dioxygenase. 5) Root phenoloxidases, which may participate in PAH-remodeling, are changed in activity in the roots colonized by mycobacterium.The project will involve the collaborative efforts of engineers and biologists at Utah State University. The undergraduates, graduates and faculty involved will be exposed to the theories and practices of both scientific disciplines. Strong involvement of minorities in the program will be supported through integration with the ADVANCE program at Utah State University recently awarded to Utah State University from NSF and GAANN grant, "Increasing the number of environmental engineering Ph.D. students at Utah State University with an emphasis on strengthening diversity." Already active in both colleges are summer placement programs for high-school students through engineering and the Biotechnology Summer Academy. Undergraduate involvement will be assisted by application to the USU Undergraduate Research Cooperative Program that will assist travel to meetings including national level conferences for presentation of data. Graduate mentorship will include regular group meetings and assistance from the USU Graduate School for tuition and for travel to national meetings. Research information will be conveyed through university publications, peer-reviewed journals and by presentations at national and international meetings. The findings will improve our abilities to predict and manage on-site phytoremediation of PAHs and will have national and international impact.

PIS：Anderson植物根-分枝杆菌的相互作用在修复多环芳烃方面是否有益？多环芳烃污染的土壤对环境和健康构成危害。植物修复是一种经济有效的现场清理方法。它非常适合于大面积的表面区域，如城市环境中被指定为棕地的区域，或难以挖掘和清除土壤的场地。这项建议侧重于更多地了解具有多环芳烃降解潜力的分枝杆菌组的生态学。目前，关于这种分枝杆菌如何与植物根相互作用，以及这种联系是否对植物和/或促进生物修复的微生物的新陈代谢产生积极影响的知识很少。需要检验的假设是：1)降解多环芳烃的分枝杆菌定植的根的存在提高了模型的生物利用度，顽固性多环芳烃，芘。2)根与分枝杆菌的相互作用增强了芘的矿化作用。3)根的定植需要分枝杆菌和根表面之间的离散相互作用。4)根的定植允许编码参与多环芳烃降解的第一种酶-双加氧酶的基因在分枝杆菌中表达。5)可能参与PAH重塑的根酚氧化酶在被分枝杆菌定植的根中的活性发生变化。该项目将涉及犹他州立大学工程师和生物学家的合作努力。参与的本科生、毕业生和教职员工将接触到这两个科学学科的理论和实践。通过与犹他州立大学最近从NSF和GAANN赠款授予犹他州立大学的高级项目的整合，将支持少数族裔大力参与该项目，“增加犹他州立大学环境工程博士生的数量，强调加强多样性。”在这两所大学中，针对高中生的工程学暑期安置项目和生物技术暑期学院都已经很活跃。本科生的参与将通过申请USU本科生研究合作项目来得到帮助，该项目将帮助旅行参加会议，包括国家层面的会议，以展示数据。研究生辅导将包括定期的小组会议和USU研究生院提供的学费和参加全国会议的旅费。研究信息将通过大学出版物、同行评议的期刊以及在国家和国际会议上的陈述来传达。这些发现将提高我们预测和管理多环芳烃现场植物修复的能力，并将对国家和国际产生影响。