Laser-induced Breakdown Spectroscopy to Characterize Individual Marine Phytoplankton

激光诱导击穿光谱法表征单个海洋浮游植物

• 批准号: 0350712
• 负责人: Robert Olson
• 金额: --
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0350712&HistoricalAwards=false
• 关键词: Laser; induced; Breakdown; Spectroscopy; Characterize

The PIs propose is to test the feasibility of developing a LIBS flow cytometer for the study of plankton populations. Ultimately the goal would be to integrate a multi-laser flow cytometer a high spectral resolution fiber optic LIBS system. Initially they will assemble a skeleton flow cytometer from components in their lab to be combined with a commercial LIBS system. Experiments will be set up to answer the following questions: 1) will the flow cell be damaged by a LIBS laser pulse powerful enough to generate the required plasma? 2) do bubbles from the plasma generation disrupt the flow so severely as to prevent subsequent measurements? and 3) can the LIBS system detect single phytoplankton cells above background seawater signals? The results will determine how to proceed with the next phase.

PI的目的是测试开发用于浮游生物种群研究的LIBS流式细胞仪的可行性。最终的目标是将多激光流式细胞仪与高光谱分辨率光纤LIBS系统集成在一起。最初，他们将在实验室中组装一个骨架流式细胞仪，与商业LIBS系统相结合。将建立实验来回答以下问题：1）流动池是否会被足够强大以产生所需等离子体的LIBS激光脉冲损坏？ 2)等离子体产生的气泡是否会严重干扰流动，从而妨碍后续测量？以及3）LIBS系统能否检测到高于背景海水信号的单个浮游植物细胞？结果将决定如何进行下一阶段。