A submersible imaging-in-flow instrument to monitor nano- and microplankton

用于监测纳米和微型浮游生物的潜水式流动成像仪器

• 批准号: 0525700
• 负责人: Robert Olson
• 金额: --
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0525700&HistoricalAwards=false
• 关键词: submersible; imaging; flow; instrument; monitor

The proposed work builds on the "FlowCytobot", a submersible flow cytometer developed by the PIs to observe phytoplankton and microzooplankton in the size range 10-100 m. This gap contains phytoplankton (including many diatoms and dinoflagellates) that can bloom in coastal waters, and microzooplanton such as protozoa that are critical to the diets of copepods and larval fish, so it is important that we monitor the organisms in this size range. The goal of this proposal, is to complete development and testing of a submersible imaging flow cytometer designed to characterize these large phytoplankton and microzooplanktonic organisms. The new instrument is a modified version of FlowCytobot that uses a combination of flow cytometric and video technology to capture high resolution (1 m) images of organisms for identification and to measure chlorophyll fluorescence associated with each image. Images will be classified by software based on a support vector machine approach, while the measurements of chlorophyll fluorescence will allow us to discriminate heterotrophic from phototrophic cells in taxa (such as dinoflagellates) that contain both kinds of members. Quantification of chlorophyll fluorescence in large phytoplankton cells will also enable patterns in bulk chlorophyll data to be better interpreted.Construction of a working version of Imaging FlowCytobot is nearly completed. The new instrument retains the anti-fouling and performance-monitoring capabilities of the original FlowCytobot; it operates autonomously but is remotely programmable and samples continuously; data will be transferred via Ethernet and fiber optic cables to a remote computer for analysis. The next steps in development involve field testing at WHOI and MVCO, optimizing the instrument on the basis of results from this testing, and refining the analysis and control software.

拟议的工作是在“FlowCytobot”的基础上进行的，这是一种由PI开发的潜水式流式细胞仪，用于观察大小在10-100米范围内的浮游植物和微型浮游动物。这一缺口包含浮游植物（包括许多硅藻和腰鞭毛虫），它们可以在沿海沃茨中大量繁殖，以及微型浮游动物，如对桡足类和幼鱼的饮食至关重要的原生动物，因此我们监测这一大小范围内的生物体非常重要。本提案的目标是完成水下成像流式细胞仪的开发和测试，该仪器旨在描述这些大型浮游植物和微型浮游动物的特征。新仪器是FlowCytobot的修改版本，它使用流式细胞仪和视频技术的组合来捕获生物体的高分辨率（1米）图像以进行识别，并测量与每个图像相关的叶绿素荧光。图像将通过基于支持向量机方法的软件进行分类，而叶绿素荧光的测量将使我们能够区分包含两种成员的类群（如甲藻）中的异养细胞和光养细胞。大型浮游植物细胞中叶绿素荧光的定量也将使大量叶绿素数据中的模式得到更好的解释。新仪器保留了原始FlowCytobot的防污和性能监测功能;它可以自动运行，但可以远程编程并连续采样;数据将通过以太网和光纤电缆传输到远程计算机进行分析。开发的下一步包括在WHOI和MVCO进行现场测试，根据测试结果优化仪器，并改进分析和控制软件。