Acquisition of equipment for direct observation of colloid attachment-detachment dynamics and direct measurement of colloid-substratum interaction and adhesion forces

0352482约翰逊该补助金提供了部分支持，用于购买两种仪器，用于直接检查胶体附着-脱离动力学，以及直接测定胶体和基质相互作用：1）一种系统，将流动池与全内反射荧光显微镜和图像分析能力（Flow-TIRFM）相结合; 2）一种电化学原子力显微镜（AFM），具有TIRFM能力。 此次收购将加强现有的研究和教育项目在胶体多孔介质中的（细菌）附着-脱离动力学，细胞间和附件分子运输途径的成像，与穿过氧化膜的电子运输相关的结构的鉴定，以及大分子与无机表面、蛋白质（或聚合物）-表面相互作用，并确定这些相互作用对与各种表面的粘附动力学的影响。 对社会的好处包括改进病原体运输模型，以确定地下水资源保护规则，改进药物输送策略，增强复合电池材料，以及改进表面处理技术。

0352482JohnsonThis grant provides partial support for the acquisition of two instruments necessary for direct examination of colloidal attachment-detachment dynamics, and direct determination of colloid and substratum interactions: 1) a system combining a flow cell with total internal reflection fluorescence microscopy and image analysis capability (Flow-TIRFM); and 2) an electrochemical atomic force microscope (AFM) with TIRFM capability. The acquisition will enhance existing research and education projects in colloidal (bacterial) attachment-detachment dynamics in porous media, imaging of intercellular and appendageal molecular transport pathways, identification of structures associated with electron transport across oxide films, and quantification of interaction of macromolecules with inorganic surfaces, protein (or polymer)-surface interactions, and determination of the effects of these interactions on adhesion dynamics with various surfaces. Benefits to society include improved pathogen transport models for determination of rules for groundwater resource protection, improved drug delivery strategies, enhanced composite battery materials, and improved surface preparation technologies.***