Acquisition of equipment for direct observation of colloid attachment-detachment dynamics and direct measurement of colloid-substratum interaction and adhesion forces

这笔拨款为购买两种仪器提供部分支持，用于直接检查胶体附着-脱离动力学，并直接确定胶体和基质的相互作用：1)将流动池与全内反射荧光显微镜和图像分析能力（flow - tirfm）相结合的系统；2)具有TIRFM功能的电化学原子力显微镜（AFM）。此次收购将加强现有的研究和教育项目，包括多孔介质中胶体（细菌）附着-脱离动力学，细胞间和附属物分子运输途径的成像，与氧化物膜上电子传输相关的结构鉴定，以及大分子与无机表面、蛋白质（或聚合物）表面相互作用的量化。并确定这些相互作用对不同表面的粘附动力学的影响。对社会的益处包括：用于确定地下水资源保护规则的病原体运输模型的改进、药物递送策略的改进、复合电池材料的增强以及表面制备技术的改进

0352482JohnsonThis grant provides partial support for the acquisition of two instruments necessary for direct examination of colloidal attachment-detachment dynamics, and direct determination of colloid and substratum interactions: 1) a system combining a flow cell with total internal reflection fluorescence microscopy and image analysis capability (Flow-TIRFM); and 2) an electrochemical atomic force microscope (AFM) with TIRFM capability. The acquisition will enhance existing research and education projects in colloidal (bacterial) attachment-detachment dynamics in porous media, imaging of intercellular and appendageal molecular transport pathways, identification of structures associated with electron transport across oxide films, and quantification of interaction of macromolecules with inorganic surfaces, protein (or polymer)-surface interactions, and determination of the effects of these interactions on adhesion dynamics with various surfaces. Benefits to society include improved pathogen transport models for determination of rules for groundwater resource protection, improved drug delivery strategies, enhanced composite battery materials, and improved surface preparation technologies.***