Equipment and Instrument Infrastructure Improvement for the MMRRC at UC Davis

加州大学戴维斯分校 MMRRC 的设备和仪器基础设施改进 摘要和工作范围 加州大学戴维斯分校突变小鼠资源和研究中心 (MMRRC) 很高兴 提交此行政补充以获得长达 1 年的支持，以响应 ORIP 参与 PA- 20-272，“现有 NIH 拨款和合作协议的行政补充”特别涉及 支持设施的改建和翻新 (A&R) 和/或购买仪器和设备 支持非艾滋病研究。本申请建议购买和安装设备以及 仪器仪表，以确保和提高畜牧、生产和生产的质量、严谨性和能力 由 MMRRC 维护和分发的突变小鼠品系的表型分析。具体来说，这个应用程序 请求资金以改善我们 MMRRC 设施的运营、可服务性、功能和能力：1) 更换损坏和无法修复的冷冻回收和分析仪器，并实现异地存储 MMRRC 种质冷冻档案库，2) 升级现有动物转运站，大幅改善 MMRRC 小鼠品系的无特定病原体 (SPF) 饲养质量，以及 3) 获得和 纳入专门的仪器和设备，以促进新的和实施的改进 肌肉骨骼、肺部和行为表型分析能力，可为研究带来实质性贡献 完整性、严谨性和可重复性。该项目的资助将完成 5 件事。首先，它将显着 提高冷冻恢复和 IVF 效率，从而加快可用性并促进快速 向提出请求的研究人员传播小鼠品系。二是加快质量控制分析。 MMRRC 小鼠品系的生殖生物学，为用户添加有关精子的宝贵信息 表型并确保它们从冷冻档案中恢复。三是质量显着提高。 为 MMRRC 小鼠提供饲养和保健服务，并提供过敏原控制以保护饲养场工作人员。 第四，它将能够在所有 MMRRC 小鼠上进行表型分析的验证和深度分析 应变，从而直接响应用户对更广泛、更全面的测试和分析的要求。 第五，它将提高生产和表型活动的严谨性、可重复性和透明度，进一步提高 支持 MMRRC 为获得全国机构批准的供应商地位而做出的努力。 总之，这些升级将显着增强我们满足 MMRRC 日益增长的期望的能力 客户获得全面验证的小鼠模型并优化生产和表型服务。 此外，由于这些升级是 MMRRC 项目特定的，因此超出了 MMRRC 提供的支持范围。 加州大学戴维斯分校校园的基本运营和维护。本补充的总体成果将是 通过改进和扩大 MMRRC 的运作，对 MMRRC 的运作产生持续而强大的影响 能够更专业地维护、管理和表征转基因小鼠品系并满足 国家生物医学研究界进行 NIH 资助的研究的需求。通过这样做，这 补充将为整个研究界带来持续的利益，并对 NIH 资助的研究。父级同行评审的 NIH 资助的赠款 (U42OD012210) 具有竞争力 续展（截至 2020 年 1 月 1 日）5 年。剩余费用请求预算为 478,325 美元（总费用） 当前资助年度（截至 2024 年 1 月 31 日）的月数少于年度直接成本预算总额的一半 家长补助金。拟议的活动均属于家长奖励的范围，其中包括归档、 表型分析，并分发研究者衍生的和中心产生的突变小鼠系。

Equipment and Instrument Infrastructure Improvement for the MMRRC at UC Davis ABSTRACT & SCOPE OF WORK The Mutant Mouse Resource and Research Center at the University of California, Davis (MMRRC) is pleased to submit this administrative supplement for up to 1 year of support in response to ORIP's participation in PA- 20-272, “Administrative Supplements to Existing NIH Grants and Cooperative Agreements” specifically related to support alterations and renovations (A&R) of facilities and/or purchase of instruments and equipment in support of non-AIDS research. This application proposes the purchase and installation of equipment and instrumentation to ensure and increase the quality, rigor, and capability of husbandry, production, and phenotyping of mutant mouse lines maintained and distributed by the MMRRC. Specifically, this application requests funds to improve operations, serviceability, functionality and capability of our MMRRC facilities by 1) replacing broken and irreparable cryorecovery and analytical instrumentation and to enable off-site storage of the MMRRC germplasm cryoarchive, 2) upgrading existing animal transfer stations to substantially improve the quality of specific pathogen-free (SPF) husbandry for MMRRC mouse strains, and 3) obtaining and incorporating specialized instrumentation and equipment to facilitate new and implement improvements in musculoskeletal, pulmonary, and behavioral phenotyping capabilities that add substantively to research integrity, rigor, and reproducibility. Funding of this project will accomplish 5 things. First, it will significantly improve cryorecovery and IVF efficiency, thus accelerating the availability and facilitating the rapid dissemination of mouse lines to requesting investigators. Second, it will expedite quality control analysis of the reproductive biology of MMRRC mouse strains, adding valuable information to users regarding sperm phenotype and ensuring their recovery from the cryoarchive. Third, it will dramatically improve the quality of husbandry and health care for MMRRC mice and provide allergen containment to protect vivarium staff. Fourth, it will enable the validation and depth of phenotyping that can be conducted on all MMRRC mouse strains, thus directly responding to users' requests for more extensive and comprehensive testing and analysis. Fifth, it will improve rigor, reproducibility, and transparency in production and phenotyping activities, further supporting efforts by the MMRRC to achieve approved vendor status by institutions across the country. Together, these upgrades will significantly enhance our ability to meet the increased expectations of MMRRC clientele for fully comprehensively validated mouse models and optimize production and phenotyping services. Further, because these upgrades are MMRRC-project specific, they are beyond the support provided by the UC Davis campus for basic operations and maintenance. The overall outcome of this supplement will be to exert a sustained and powerful influence on operations of the MMRRC by improving and expanding its capability to more expertly maintain, manage, and characterize genetically-modified mouse strains and meet the needs of the national biomedical research community conducting NIH-funded research. By doing so, this supplement will provide a sustained benefit to the overall research community and have a positive impact on NIH-funded research. The parent peer-reviewed NIH funded grant (U42OD012210) has been competitively renewed (as of January 1, 2020) for 5 years. A budget of $478,325 (total costs) requested for the remaining months of the current grant year (until January 31, 2024) is less than half the annual total direct cost budget of the parent grant. The proposed activities all fall within the scope of the parent award, which includes archiving, phenotyping, and distributing investigator-derived and Center-produced mutant mouse lines.