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Role of the magnetosome proteins MamGFDC in biomineralization and size control of magnetite crystals in Magnetospirillum gryphiswaldense

磁小体蛋白 MamGFDC 在 Magnetospirillum gryphiswaldense 磁铁矿晶体生物矿化和尺寸控制中的作用

基本信息

批准号：
165534956
负责人：
Professor Dr. Dirk Schüler
金额：
--
依托单位：
Lehrstuhl für Mikrobiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/165534956?language=en
关键词：
Role magnetosome proteins MamGFDC biomineralization

项目摘要

Bacterial magnetosomes are unique prokaryotic organelles consisting of membrane-bounded magnetite nanocrystals that are synthesized for magnetic navigation. One major unsolved question is how magnetosome biomineralization is precisely regulated to achieve the structural perfection, characteristic shapes and uniform sizes of magnetite nanocrystals. The abundant magnetosome-associated MamGFDC proteins in the bacterium Magnetospirillum gryphiswaldense represent the first biological determinants implicated in size control of magnetosomes, although their precise functions are still unknown. We will characterize MamGFDC and the related MmsF protein and elucidate the mechanism by which they interfere with magnetite biomineralization. By genetic analysis we will estimate their individual contributions to size control. Controlled mamGFDC expression will then be used for the synthesis of size-modified magnetic nanoparticles. The intracellular localization and interaction of wild-type and mutant proteins will be studied by biochemical methods and fluorescence microscopy. The potential to bind magnetite and to directly interfere with its crystallization will be assessed in vitro. Our studies may facilitate the biogenic synthesis of tailored magnetic nanoparticles for biotechnological applications and may also contribute to an improved understanding of biomolecular interactions at the organic/inorganic interface of iron biominerals.

细菌磁小体是一种独特的原核生物细胞器，由磁性纳米晶体组成。一个主要的未解决的问题是如何精确地调节磁小体生物矿化，以实现磁铁矿纳米晶体的结构完美，特征形状和均匀尺寸。在细菌Magnetocellum gryphiswaldense中丰富的磁小体相关MamGFDC蛋白代表了涉及磁小体大小控制的第一个生物决定因素，尽管它们的精确功能仍然未知。我们将表征MamGFDC和相关的MmsF蛋白，并阐明它们干扰磁铁矿生物矿化的机制。通过遗传分析，我们将估计它们各自对大小控制的贡献。然后将受控的mamGFDC表达用于合成尺寸改性的磁性纳米颗粒。将通过生物化学方法和荧光显微镜研究野生型和突变蛋白的细胞内定位和相互作用。将在体外评估结合磁铁矿和直接干扰其结晶的可能性。我们的研究可能有助于生物合成的定制的磁性纳米粒子的生物技术应用，也可能有助于提高对铁生物矿物的有机/无机界面的生物分子相互作用的理解。