GOALI: Development of Combinatorial Polymeric Substrates for Efficient Screening of Protein Adsorption

目标：开发组合聚合物基质以有效筛选蛋白质吸附

• 批准号: 0403535
• 负责人: Jan Genzer
• 金额: --
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0403535&HistoricalAwards=false
• 关键词: GOALI; Development; Combinatorial; Polymeric; Substrates

Proposal Title: GOALI: Development of Combinatorial Polymeric Substrates for Efficient Screening of Protein AdsorptionProposal Number: CTS-0403535Principal Investigator: Jan GenzerInstitution: North Carolina State UniversityThe objective of this proposal is to develop a novel technology for investigating protein adsorption on material surfaces. A combinatorial screening methodology that improves upon existing analytical tools will enable faster, more reliable, and more efficient evaluation of protein adsorption. This approach will provide an effective analytical method and will facilitate manufacturing of devices with controllably modified biointerfacial properties. Novel polymeric surfaces based on the tailored deposition of macromolecules grafted to flat silica substrates will be fabricated and characterized. Conditions will be controlled to permit formation of continuous gradients in polymer grafting densities, and graft-polymer chain length. Gradient structures, on which the polymer grafting density and chain length of the grafted polymers vary independently in orthogonal directions, will be prepared. In terms of the broader impacts, educational efforts will focus on activities in an elementary school in Mount Airy, NC aimed at attracting local K-12 teachers and students. The gradient-based technology developed in this program will provide a powerful methodology that should be superior to current techniques in that it enables fast and simple screening of protein adsorption and complete and reliable exploration of a broad parameter space. The structures developed in this program will facilitate controlled adsorption of cells, a process that represents the central focus of many pharmaceutical devices.

提案标题：目标：开发用于蛋白质吸附高效筛选的组合聚合物基质提案编号：CTS-0403535主要研究者：Jan Genzer机构： 北卡罗来纳州州立大学本提案的目的是开发一种新的技术，研究蛋白质吸附在材料表面。改进现有分析工具的组合筛选方法将能够更快、更可靠和更有效地评估蛋白质吸附。这种方法将提供一种有效的分析方法，并将有助于制造具有可控改性生物界面特性的器械。 新型聚合物表面的基础上定制沉积的大分子接枝到平面二氧化硅基板将被制造和表征。 将控制条件以允许形成聚合物接枝密度和接枝聚合物链长的连续梯度。将制备梯度结构，其上的聚合物接枝密度和接枝聚合物的链长在正交方向上独立地变化。 就更广泛的影响而言，教育工作将集中在北卡罗来纳州艾里山一所小学的活动上，旨在吸引当地K-12教师和学生。 该计划中开发的基于梯度的技术将提供一种强大的方法，该方法应上级当前技术，因为它可以快速简单地筛选蛋白质吸附，并完整可靠地探索广泛的参数空间。该计划中开发的结构将促进细胞的受控吸附，这一过程代表了许多制药设备的中心焦点。