Research Starter Grant: Assessing the Role of the Environmental Stress Response in Protecting Yeast Cells Against Diverse Stressful Conditions

研究启动资金：评估环境应激反应在保护酵母细胞免受多种应激条件影响中的作用

• 批准号: 0404603
• 负责人: Audrey Gasch
• 金额: $ 5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0404603&HistoricalAwards=false
• 关键词: Research; Starter; Grant; Assessing; Role

The Environmental Stress Response (ESR) is a large gene expression program that is initiated when cells are exposed to stress. Although the response has been observed in many gene expression studies, the role of the program is still unknown. It has been hypothesized that the ESR is involved in acquired stress resistance, in which cells that are exposed to a low dose of stress become resistant to an otherwise-lethal dose of a second unrelated stress. This project will test the role of the ESR in acquired stress resistance in yeast. The first phase of the project will use molecular biology techniques to characterize the determinants of acquired stress resistance, including the specificity of acquired resistance, the required doses of initial stress exposure, and the kinetics of the response. The second phase of the project will use DNA microarrays and computational analysis to identify gene expression changes that correlate temporally with the kinetics of acquired stress resistance. Finally, the role that ESR plays in this phenomenon will be tested by assessing the ability of a mutant strain defective in ESR initiation to acquire stress resistance.

环境应激反应（ESR）是一个大型基因表达程序，当细胞暴露于压力时启动。 尽管在许多基因表达研究中都观察到了这种反应，但该程序的作用仍然未知。 据推测，ESR 与获得性应激抵抗有关，即暴露于低剂量应激的细胞对第二种不相关应激的致命剂量具有抵抗力。 该项目将测试 ESR 在酵母获得性应激抵抗中的作用。 该项目的第一阶段将利用分子生物学技术来表征获得性应激抵抗的决定因素，包括获得性抵抗的特异性、初始应激暴露所需的剂量以及反应动力学。 该项目的第二阶段将使用 DNA 微阵列和计算分析来识别与获得性应激抵抗动力学在时间上相关的基因表达变化。 最后，将通过评估 ESR 启动缺陷的突变菌株获得抗逆性的能力来测试 ESR 在这一现象中所起的作用。