Arabidopsis2010: Determining Genome-wide Transcription Networks of TGA Factors

拟南芥2010：确定 TGA 因子的全基因组转录网络

• 批准号: 0404746
• 负责人: Christopher Town
• 金额: $ 155.26万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0404746&HistoricalAwards=false
• 关键词: Arabidopsis2010; Determining; Genome; wide; Transcription

The completed genome sequence of Arabidopsis thaliana codes for at least 1,533 transcriptional regulators, of which the majority are predicted to bind DNA. Interactions between these DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of batteries of genes that effect homeostasis, development and adaptation. However, our present knowledge of target genes and regulatory networks for all but a few plant transcription factors remains rudimentary at best. Considering the challenge ahead, efforts to assign gene-regulatory functions to the relatively large number of uncharacterized transcription factors of this higher plant by 2010 will strongly benefit from more global and high-throughput approaches. In this regard, this 2010 Project award will make use of a complementary and integrated strategy, involving high-throughput plant chromatin immunoprecipitation (pChIP) and gene expression analysis, for mapping transcriptional regulatory networks of Arabidopsis. Additional parallel experiments are planned to assess the functional validity of findings. The focus of study is the TGA family of basic/leucine-zipper proteins, which comprise a complex transcriptional system with important regulatory functions in development and defense. A key question to be answered here is the identity of primary target genes and their associated networks. Data from this project will be posted periodically under links to be established on TIGR (http://www.tigr.org/tdb/e2k1/ath1/) and CAB (http://www.umbi.umd.edu/~cab/) websites. Methodologies and tools to be developed under this award will include a high-throughput pChIP technology and partial genome array of intergenic sequences, which will be made publicly available as they come on line during the 3 year time-line of this project. In addition to developing a new paradigm for understanding transcriptional control mechanisms in Arabidopsis, this project will promote education and training missions that are encouraged by NSF.The goal of this 2010 project award is to identify and categorize gene-regulatory interactions that occur between a subset of DNA-binding proteins and the genes that they govern in the living plant. Expression of these genes is implicated in defense responses by plants to infection and toxins. Thus, this work will not only provide important new information on the mechanistic basis of how genes are regulated, but also may benefit agriculture by leading to new environmentally-friendly strategies for genetically enhancing natural protective genes of crop plants.

完整的拟南芥基因组序列编码至少1，533个转录调节因子，其中大多数被预测与DNA结合。 这些DNA结合蛋白和同源启动子序列之间的相互作用是建立影响稳态、发育和适应的基因组的空间和时间表达模式的主要决定因素。 然而，我们目前的知识目标基因和调控网络的所有，但少数植物转录因子仍然是最基本的。 考虑到未来的挑战，到2010年，将基因调控功能分配给相对大量的这种高等植物的未表征的转录因子的努力将大大受益于更多的全球性和高通量的方法。 在这方面，这个2010年的项目奖将利用一个互补的和综合的战略，涉及高通量的植物染色质免疫沉淀（pChIP）和基因表达分析，映射转录调控网络的拟南芥。 计划进行额外的平行实验，以评估结果的功能有效性。 研究的重点是碱性/亮氨酸拉链蛋白的TGA家族，其包括在发育和防御中具有重要调节功能的复杂转录系统。 这里要回答的一个关键问题是主要靶基因及其相关网络的身份。 该项目的数据将定期张贴在TIGR（http：//www.tigr.org/tdb/e2k1/ath1/）和CAB（http：//www.umbi.umd.edu/cab/）网站上建立的链接上。 根据该奖项开发的方法和工具将包括高通量pChIP技术和基因间序列的部分基因组阵列，这些技术和工具将在该项目的3年时间线内上线时公开提供。 除了开发一个新的模式，了解转录控制机制，在拟南芥，该项目将促进教育和培训任务，鼓励由NSF.这个2010年的项目奖的目标是确定和分类基因调控之间发生的相互作用的一个子集的DNA结合蛋白和基因，他们在活的植物管理。 这些基因的表达与植物对感染和毒素的防御反应有关。 因此，这项工作不仅将提供重要的新信息的机制基础上的基因是如何调节的，但也可能有利于农业，导致新的环境友好的战略，遗传增强作物的天然保护基因。