Arabidopsis 2010: Completing the Expression Catalog of the Arabidopsis Transcriptome by Quantitative Real Time PCR

几个大规模的项目已经产生了模式植物拟南芥基因组中27，000多个基因中大约23，000个基因的表达数据。然而，剩余的4,000多个基因的表达水平无法通过迄今为止使用的方法可靠地检测到，并且对这组基因的功能知之甚少。该项目的目的是为这些4，000多个拟南芥基因在各种组织和条件下开发一个定量表达谱目录，这些基因要么低于AffyteATH 1阵列可检测的水平，要么使用高通量定量实时PCR在阵列上不显示，该技术比以前的方法灵敏得多。到该项目结束时，拟南芥和植物研究团体将在各种实验条件下获得拟南芥基因组中基本上每个基因的基因表达谱，这是2010年项目目标的一个重要里程碑。将这4000多个低表达基因的表达模式与较大数据集中已有的约23，000个更好表征的基因进行比较，将为这组知之甚少的基因的生物学功能提供新的见解。同时，在较小的规模上，将使用敏感的报告基因构建体确定这些基因中约1，000个基因的空间表达谱，从而进一步了解它们在植物中的作用。该项目的说明可在http：//www.tigr.org/tdb/e2k1/ath1/qpcr/上查阅;基因表达数据在处理后将张贴在该网站上，并张贴在两个社区数据库TAIR（www.arabidopsis.org）和NASC（www.arabidopsis.info）上。本项目开发的分子和生物资源将通过拟南芥生物储备中心（http：//www.biosci.ohio-state.edu/abrplantbio/Facilities/abrc/abrchome.htm）分发。在这项工作的过程中，本科生和高中教师将在实验室主持，为前者提供宝贵的实践研究经验，后者对材料有足够的熟悉程度，能够将他们的工作转化为学生的新课堂课程。

Several large-scale projects have generated expression data for approximately 23,000 of the 27,000+ genes in the genome of the model plant Arabidopsis thaliana. However, the remaining 4,000+ genes are expressed at levels that are not reliably detected by the methods used to date and very little is known about the functions of this set of genes. The aim of this project is to develop a catalog of quantitative expression profiles under a variety of tissues and conditions for these 4,000+ Arabidopsis genes that either fall below levels detectable by the Affymetrix ATH1 array or are not represented on the array using high throughput quantitative real-time PCR, a technique that is much more sensitive than previous methods. By the conclusion of this project, the Arabidopsis and plant research communities will have gene expression profiles for essentially every gene in the Arabidopsis genome under a wide variety of experimental conditions, a major milestone in the goals of the 2010 project. Comparison of the expression patterns of these 4000+ low-expressed with the approximately 23,000 better characterized genes already available in the larger data sets will provide new insights into the biological functions of this set of poorly understood genes. In parallel, and on a smaller scale, the spatial expression profiles will be determined for ~ 1,000 of these genes using sensitive reporter gene constructs, thus providing further insight into their roles in the plant. A description of the project is available at http://www.tigr.org/tdb/e2k1/ath1/qpcr/; the gene expression data will be posted on that web site as it is processed, as well as at two community databases, TAIR (www.arabidopsis.org) and NASC (www.arabidopsis.info). Molecular and biological resources developed in this project will be distributed through the Arabidopsis Biological Stock Center (http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/abrchome.htm). During the course of this work, undergraduates and high school teachers will be hosted in the laboratory, to provide the former with valuable hands-on research experience and the latter with sufficient familiarity with the materials to be able to translate their work into new classroom programs for their students.