Regulation of Neurohormone Synthesis in Aplysia
海兔神经激素合成的调节
基本信息
- 批准号:0414493
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-09-01 至 2008-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Neurohormones are responsible for controlling different aspects of physiology and behavior including body growth, eating, drinking, and sex and reproduction. The broad goal of this project is to understand cellular mechanisms controlling synthesis of a neuropeptide hormone using neurons that control reproduction in the mollusk Aplysia. Earlier studies from the PI's laboratory showed that electrical excitation of the reproductive neuroendocrine bag-cell neurons (BCNs) led to large amounts of secretion of the neuropeptide egg-laying hormone (ELH). It was revealed that electrical excitation upregulates ELH synthesis to replenish releasable stores of hormone by both sodium- and calcium-dependent pathways that are independent from both ELH secretion and action potential firing. Furthermore, a novel protein-synthetic pathway involving a special code on ELH mRNA called an internal ribosomal entry site (IRES) plays an important role in mediating the effects of electrical excitation on upregulating specifically ELH synthesis, at the expense of non-essential proteins in the cell. This is similar to how viruses control protein synthesis in cells following infection, and was not thought to play a physiological role in cellular systems. The aim of this project is to understand the mechanistic links between membrane electrical excitation, sodium/calcium ion fluxes into the bag cell neurons, IRES activation on ELH mRNA, and ELH synthesis. Experiments will utilize electrophysiological monitoring of membrane electrical excitability and optical imaging of intracellular calcium concentrations in response to treatments that alter sodium/calcium flux, biochemical measurement of ELH synthesis, and a combination of molecular biological tools and optical imaging to monitor IRES activation in living bag cell neurons in the intact Aplysia nervous tissue. The outcome from the proposed experiments will lead to entirely novel conclusions regarding the control of specific protein synthesis in cells. This project provides excellent training opportunities for graduate and undergraduate students embarking on cutting-edge studies using an integrative experimental approach
神经激素负责控制生理和行为的不同方面,包括身体生长、饮食、性和生殖。该项目的主要目标是了解利用控制软体动物繁殖的神经元控制神经肽激素合成的细胞机制。PI实验室早期的研究表明,生殖神经内分泌袋细胞神经元(bcn)的电刺激导致神经肽产卵激素(ELH)的大量分泌。结果表明,电刺激通过钠依赖性和钙依赖性途径上调ELH合成,补充可释放的激素储备,而这些途径不依赖于ELH分泌和动作电位放电。此外,一种新的蛋白质合成途径涉及ELH mRNA上的特殊编码,称为内部核糖体进入位点(IRES),在介导电激发对特异性ELH合成上调的影响中起重要作用,而牺牲细胞中的非必需蛋白质。这类似于病毒在感染后控制细胞内蛋白质合成的方式,并且不被认为在细胞系统中起生理作用。本项目的目的是了解膜电兴奋、钠/钙离子进入袋细胞神经元、ELH mRNA上IRES激活和ELH合成之间的机制联系。实验将利用膜电兴奋性的电生理监测和细胞内钙浓度的光学成像来响应改变钠/钙通量的处理,ELH合成的生化测量,以及分子生物学工具和光学成像的组合来监测完整的海兔神经组织中活袋细胞神经元的IRES激活。所提出的实验结果将导致关于控制细胞中特定蛋白质合成的全新结论。本项目采用综合实验方法,为研究生和本科生开展前沿研究提供了良好的培训机会
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Nancy Wayne其他文献
Nancy Wayne的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Nancy Wayne', 18)}}的其他基金
Conference on Neural Control of Behavior, University of California, February 9-12, 2006
行为神经控制会议,加利福尼亚大学,2006 年 2 月 9-12 日
- 批准号:
0511838 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Standard Grant
Conference on Neural Control of Behavior, February 5-8, 2004, at UCLA Los Angeles, CA
行为神经控制会议,2004 年 2 月 5-8 日,加利福尼亚州洛杉矶加州大学洛杉矶分校
- 批准号:
0314962 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Standard Grant
SGER: Role of CREB in Regulating Neuropeptide Synthesis
SGER:CREB 在调节神经肽合成中的作用
- 批准号:
0314701 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Standard Grant
Conference on Neural Control of Behavior: Los Angeles, CA: March 14 -17, 2002
行为神经控制会议:加利福尼亚州洛杉矶:2002 年 3 月 14 日至 17 日
- 批准号:
0116834 - 财政年份:2001
- 资助金额:
-- - 项目类别:
Standard Grant
POWRE: Effects of Cytokines on Neuroendocrine Physiology
POWRE:细胞因子对神经内分泌生理学的影响
- 批准号:
9805789 - 财政年份:1998
- 资助金额:
-- - 项目类别:
Standard Grant
相似海外基金
Mode of action and role of neurohormone in the regulation of reproductive functions and its application for artificial seed production in major bivalves
神经激素在主要双壳类生殖功能调节中的作用方式和作用及其在人工制种中的应用
- 批准号:
16H04978 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (B)
Plant peptides as modulators of invertebrate neurohormone receptors
植物肽作为无脊椎动物神经激素受体的调节剂
- 批准号:
FT140100730 - 财政年份:2015
- 资助金额:
-- - 项目类别:
ARC Future Fellowships
Neurohormone cascades during crustacean ecdysis
甲壳动物蜕皮过程中的神经激素级联
- 批准号:
BB/E023126/1 - 财政年份:2007
- 资助金额:
-- - 项目类别:
Research Grant
Natrecor and Furosemide on Renal & Neurohormone Response
Natrecor 和呋塞米对肾脏的影响
- 批准号:
7040801 - 财政年份:2004
- 资助金额:
-- - 项目类别:
ADULT NEUROHORMONE MECHANISM RESPONSE TO LARVAL CROWDING
成虫神经激素机制对幼虫拥挤的反应
- 批准号:
2854171 - 财政年份:1996
- 资助金额:
-- - 项目类别:
Control of Neurohormone Release at Nerve Endings
控制神经末梢的神经激素释放
- 批准号:
9410834 - 财政年份:1994
- 资助金额:
-- - 项目类别:
Continuing Grant