Characterization of Fast Dissociation Pathways of Polyatomic Ions

多原子离子快速解离途径的表征

• 批准号: 0416338
• 负责人: Vicki Wysocki
• 金额: $ 52.1万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0416338&HistoricalAwards=false
• 关键词: Characterization; Fast; Dissociation; Pathways; Polyatomic

Professor Vicki Wysocki of the University of Arizona is supported by the Analytical and Surface Chemistry Program to study the fast fragmentation of peptides. The fundamental question in this work is one that asks why we are able to see significant fragmentation on the timescale of the typical mass spectrometry experiment, while the best theory available suggests that fragments should only be observed with longer timescale MS experiments. The overall hypothesis is that there are fast fragmentation pathways (via previously undescribed reactive intermediates) that lead to the appearance of products on timescales that allow observation by mass spectrometers with microsecond or shorter experimental timeframes. The PI uses surface collisions in combination with matrix-assisted laser desorption ionization (and later electrospray ionization) and time-of-flight mass spectrometry to probe these processes. The question is studied by varying both the nature of the surface and the nature of the projectiles. There is a well-established collaboration with the Futrell group at PNNL and the theorist Hase at Texas Tech. Students are well-trained in physical-analytical chemistry and mass spectrometry.Fragmentation of peptides is commonly used in proteomics, in which mass spectrometry plays a critical role. Understanding fragmentation at a fundamental level can lead to more efficient analytical instruments, and predictive models for database purposes.

亚利桑那大学的Vicki Wysocki教授得到了分析和表面化学项目的支持，研究肽的快速片段化。 这项工作的基本问题是，为什么我们能够在典型的质谱实验的时间尺度上看到显着的碎片化，而现有的最佳理论表明，碎片只应该在更长的时间尺度的MS实验中观察到。总体假设是，存在快速裂解途径（通过先前未描述的反应性中间体），其导致产物在允许质谱仪以微秒或更短的实验时间范围观察的时间尺度上出现。PI使用表面碰撞结合基质辅助激光解吸电离（和后来的电喷雾电离）和飞行时间质谱来探测这些过程。通过改变表面的性质和射弹的性质来研究这个问题。与PNNL的Futrell小组和德克萨斯理工大学的理论家Hase有着良好的合作关系。 学生在物理分析化学和质谱学方面有良好的训练。多肽的片段化通常用于蛋白质组学，其中质谱学起着关键的作用。从根本上理解碎片化问题，可以导致更有效的分析工具和用于数据库目的的预测模型。