A Q-TOF Tandem Mass Spectrometer with SID and CID Activation Methods

具有 SID 和 CID 激活方法的 Q-TOF 串联质谱仪

• 批准号: 0244437
• 负责人: Vicki Wysocki
• 金额: $ 52.58万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0244437&HistoricalAwards=false
• 关键词: TOF; Tandem; Mass; Spectrometer; SID

A grant has been awarded to Dr. Vicki Wysocki at the University of Arizona to design and construct an improved quadrupole-time-of-flight (Q-TOF) tandem mass spectrometer (MS-MS) designed to include two complementary activation methods, surface-induced dissociation (SID; surface target) and collision-induced dissociation (CID; gaseous target). Two biological measurement challenges that will be addressed by the proposed instrument are (1) protein identification from complex mixtures with improvements in numbers of total correct identifications and (2) analysis of glycoconjugates, specifically glycosphingolipids, from complex mixtures without prior deglycosylation. The proposed instrument will consist of the following components: a combination ion source (atmospheric MALDI and micro/nanoESI); a quadrupole mass analyzer to select ions of interest for activation; a surface for surface-induced dissociation; a hexapole for collision-induced dissociation or for trapping of surface-activated ions; and an orthogonal TOF chamber for analysis of product ions produced by CID or SID. This project will result in an instrument with several enhanced capabilities and in an excellent training experience for project members. The instrument will provide high sensitivity, flexibility in ion activation, a high duty cycle, high resolution and high mass accuracy for product ions, and fast acquisition of spectra (compatible with microcapillary LC introduction into the ESI source). Successful development of this instrument will have a significant impact on proteomics research.

亚利桑那大学的Vicki Wysocki博士获得了一笔赠款，用于设计和建造改进的四极杆飞行时间（Q-TOF）串联质谱仪（MS-MS），该质谱仪设计包括两种互补的活化方法，即表面诱导解离（SID;表面靶）和碰撞诱导解离（CID;气体靶）。 所提出的仪器将解决两个生物测量挑战：（1）从复杂混合物中鉴定蛋白质，提高总正确鉴定的数量;（2）从复杂混合物中分析糖缀合物，特别是鞘糖脂，而无需事先去糖基化。 拟议的仪器将包括以下组成部分：（大气MALDI和微/纳ESI）;四极质量分析器，以选择感兴趣的离子用于活化;表面诱导解离;六极，用于碰撞诱导解离或用于捕获表面活化的离子;以及用于分析由CID或SID产生的产物离子的正交TOF室。 该项目将产生一种具有若干增强功能的仪器，并为项目成员提供极好的培训经验。该仪器将提供高灵敏度、离子活化灵活性、高占空比、高分辨率和产物离子的高质量准确度，以及光谱的快速采集（与微毛细管LC引入ESI源兼容）。 该仪器的成功开发将对蛋白质组学研究产生重大影响。