A Q-TOF Tandem Mass Spectrometer with SID and CID Activation Methods

亚利桑那大学的Vicki Wysocki博士获得了一笔拨款，用于设计和构建改进的四极飞行时间（Q-TOF）串联质谱仪（MS-MS），该质谱仪包括两种互补的激活方法，表面诱导解离（SID；表面目标）和碰撞诱导解离（CID；气体目标）。该仪器将解决的两个生物测量挑战是：(1)从复杂混合物中鉴定蛋白质，提高总正确鉴定的数量；(2)从复杂混合物中分析糖缀合物，特别是鞘糖脂，没有事先去糖基化。拟议的仪器将由以下部分组成：组合离子源（大气MALDI和微/纳米esi）；四极杆质量分析仪，用于选择感兴趣的离子进行活化；用于表面诱导解离的表面；用于碰撞诱导解离或捕获表面活化离子的六极体；以及用于分析CID或SID产生的产物离子的正交TOF室。该项目将产生一种具有多种增强功能的仪器，并为项目成员提供出色的培训经验。该仪器将提供高灵敏度，灵活的离子活化，高占空比，高分辨率和高质量精度的产物离子，并快速获取光谱（兼容微毛细管LC引入ESI源）。该仪器的成功开发将对蛋白质组学研究产生重大影响。

A grant has been awarded to Dr. Vicki Wysocki at the University of Arizona to design and construct an improved quadrupole-time-of-flight (Q-TOF) tandem mass spectrometer (MS-MS) designed to include two complementary activation methods, surface-induced dissociation (SID; surface target) and collision-induced dissociation (CID; gaseous target). Two biological measurement challenges that will be addressed by the proposed instrument are (1) protein identification from complex mixtures with improvements in numbers of total correct identifications and (2) analysis of glycoconjugates, specifically glycosphingolipids, from complex mixtures without prior deglycosylation. The proposed instrument will consist of the following components: a combination ion source (atmospheric MALDI and micro/nanoESI); a quadrupole mass analyzer to select ions of interest for activation; a surface for surface-induced dissociation; a hexapole for collision-induced dissociation or for trapping of surface-activated ions; and an orthogonal TOF chamber for analysis of product ions produced by CID or SID. This project will result in an instrument with several enhanced capabilities and in an excellent training experience for project members. The instrument will provide high sensitivity, flexibility in ion activation, a high duty cycle, high resolution and high mass accuracy for product ions, and fast acquisition of spectra (compatible with microcapillary LC introduction into the ESI source). Successful development of this instrument will have a significant impact on proteomics research.