Signaling to Cell Morphogenesis in Arabidopsis Leaves

拟南芥叶细胞形态发生的信号传导

• 批准号: 0417255
• 负责人: Zhenbiao Yang
• 金额: $ 39万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0417255&HistoricalAwards=false
• 关键词: Signaling; Cell; Morphogenesis; Arabidopsis; Leaves

The long-term goal of this project is to understand how plant cells achieve various shapes. Cell shape formation is important for the development and the morphogenesis of plants, but the molecular mechanism underlying this fundamental process is poorly understood. This project focuses on a network of regulatory proteins in the control of interlocking jigsaw puzzle appearance of Arabidopsis leaf epidermal cells. A central player in this network is a plant-specific guanine nucleotide binding protein known as ROP. The formation of jigsaw puzzle appearance requires the coordination of localized cellular outgrowth in the lobe-forming region of the cell with localized constraint of outgrowth in the other regions. ROP promotes lobing by activating the RIC4 protein to assemble the actin cytoskeleton required for localized cell outgrowth, as well as by inactivating the RIC1 protein that organizes the microtubule cytoskeleton for constraining cellular outgrowth. The RIC1 pathway feedback represses ROP activation to assure the formation of indentations. In this proposed work, genetic, molecular, cell biological, and proteomic approaches will be used to elucidate the mechanism by which ROP activity is regulated as well as the mechanism underpinning the RIC1 regulation of microtubules. This research aim is expected to provide new insights into the mechanism for cell shape formation in plants and to identify new genes that control cell morphogenesis and plant development. These genes may prove to be useful for genetically manipulating plant forms that are beneficial to agricultural and horticultural practices. This project will also integrate research with training of undergraduate and graduate students and participation of underrepresented undergraduate students through outreach programs such as Minority Student Summer Research Internships and REU Programs.

该项目的长期目标是了解植物细胞如何实现各种形状。细胞形状的形成对于植物的发育和形态建成是重要的，但这一基本过程的分子机制却知之甚少。 本项目主要研究拟南芥叶表皮细胞中的调控蛋白网络。 在这个网络中的一个中心球员是一种植物特异性鸟嘌呤核苷酸结合蛋白，称为ROP。 拼图外观的形成需要细胞的叶形成区域中的局部细胞生长与其他区域中的生长的局部约束的协调。ROP通过激活RIC 4蛋白来组装局部细胞生长所需的肌动蛋白细胞骨架，以及通过灭活RIC 1蛋白来组织微管细胞骨架以限制细胞生长，从而促进成瓣。 RIC1通路反馈抑制ROP活化以确保形成凹陷。 在这项拟议的工作中，遗传，分子，细胞生物学和蛋白质组学的方法将被用来阐明ROP活性的调节机制，以及RIC 1的微管调节机制的基础。 本研究旨在为植物细胞形态形成机制提供新的见解，并发现控制细胞形态建成和植物发育的新基因。 这些基因可能被证明对于遗传操纵对农业和园艺实践有益的植物形态有用。 该项目还将通过少数民族学生暑期研究实习和REU计划等外联计划，将研究与本科生和研究生的培训以及代表性不足的本科生的参与相结合。