Arabidopsis 2010: Development of Recombinant Inbred Lines for QTL Analysis

野生拟南芥菌株是一个丰富的天然遗传变异的来源不存在于常见的实验室菌株。 利用这种变异的一种方法是将野生种群纳入称为重组近交系（RIL）的遗传作图种群中。 这些线使研究人员能够精细地绘制影响任何可量化性状的所有植物基因。 绘制这些数量性状基因座（QTL）表明有多少基因影响一个性状，以及每个基因的作用有多强，并将它们放在遗传图谱上。 定位必须先于基因的分子鉴定，这是拟南芥2010计划的主要目标。 Alan Lloyd的项目将构建五个新的RIL群体（每个群体300个品系，每个品系200个标记）。 所有这些都将在俄亥俄州州立大学的拟南芥生物资源中心公开。 测绘数据将通过拟南芥信息资源（TAIR）在线。 头两套中密度地图将于2004年提供，最后三套将于2005年提供。 高密度图谱将于2005年和2006年发布。拟南芥QTL分析将有助于理解作物产量、抗病性和耐旱性等性状的遗传。 这项研究为本科生和研究生提供了学习现代生物技术和创造性思维的机会，并强烈鼓励本科生获得第一手的实验室经验。

Wild Arabidopsis strains are a rich source of natural genetic variation not present in common laboratory strains. One way to exploit this variation is to incorporate wild stocks into genetic mapping populations called Recombinant Inbred Lines (RIL). These lines allow researchers to finely map all the plant genes affecting any quantifiable trait. Mapping these Quantitative Trait Loci (QTL) indicates how many genes affect a trait and how strongly each gene acts, and places them on a genetic map. Mapping must precede the molecular identification of the genes, a main goal of the Arabidopsis 2010 initiative. Alan Lloyd's project will construct five new RIL populations (300 lines per population, 200 markers per line). All will be publicly available from the Arabidopsis Biological Resource Center at Ohio State University. Mapping data will be on-line through The Arabidopsis Information Resource (TAIR). The first two sets of medium-density maps will be available in 2004, the last three in 2005. High density maps will be released in 2005 and 2006.QTL analysis in Arabidopsis thaliana will contribute to understanding the genetics of such crop traits as yield, disease resistance, and drought tolerance. This research provides opportunities for undergraduate and graduate students to learn modern biotechnology and creative thinking, and undergraduate students are strongly encouraged to get first-hand lab experience.