Arabidopsis 2010: A Systematic Approach to Automated Production of Recombinant Inbred Lines

该项目的目标是为科学界开发一种资源，促进自然遗传变异的广泛使用，以实现2010年项目目标，即为每个拟南芥基因分配一个功能。至少将产生四套新的定位重组自交系(RIL)。来自数百个可用野生型材料的潜在亲本对将被系统地筛选，以进行遗传和表型差异以及成对距离的筛选。被选中的亲本将包括以前从未开发过的野生品系。将尽可能多地使用简单序列长度DNA多态(SSLP)进行作图。来自400个RI线组的95个个体将被绘制在100个座位上，生成密度约为6厘米的地图。所有RIL的种子将通过拟南芥生物资源中心(http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/ABRCHOME.HTM)公开提供，地图和地图数据将在2004年1月之前通过拟南芥信息资源(http://www.arabidopsis.org/))在线提供。在这个项目的过程中，本科生、研究生和技术人员将接受机器人学、分子生物学、遗传学和数量性状图谱方面的培训。此外，奥斯汀社区学院生物技术项目的学生将通过外展项目接受类似的培训。在少数拟南芥实验室菌株中使用常规遗传学，将无法达到2010年计划的目标，即完全了解每一种植物基因的功能。该项目将提供一种方法来定位和识别当地野生拟南芥种群中的基因，这些基因对于适应许多不同的环境非常重要，并且是许多不同进化史的结果。这些不同的环境将包括不同的土壤类型、日长、害虫种群、湿度水平、温度和其他因素。这最终将导致更好地理解重要的适应措施，以便纳入世界农作物。

The goal of this project is to develop a resource for the scientific community that promotes expanded use of natural genetic variation in reaching the 2010 Project objective of assigning a function to each Arabidopsis gene. At least four new sets of mapped Recombinant Inbred Lines (RILs) will be produced. Potential pairs of parents from hundreds of available wild type accessions will be systematically screened for genetic and phenotypic variance and pairwise distances. The chosen parents will include wild lines not previously exploited. Simple Sequence Length DNA Polymorphisms (SSLPs) will be used as much as possible for mapping. Ninety-five individuals, from sets of 400 RI lines will be mapped at one hundred loci, generating a map with a density of approximately 6 centiMorgans. Seeds for all RILs will be publicly available through the Arabidopsis Biological Resource Center (http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/ABRCHOME.HTM) and maps and mapping data will be available online through The Arabidopsis Information Resource (http://www.arabidopsis.org/) by January 2004. During the course of this project, undergraduate and graduate students and technicians will be trained in robotics, molecular biology, genetics, and quantitative trait mapping. In addition, students in the Biotechnology Program at the Austin Community College will receive similar training through an outreach program. Conventional genetics used in a small number of laboratory strains of Arabidopsis, will not be able to reach the 2010 Project goal of complete knowledge of every plant gene's function. This project will provide a way to map and identify genes in local wild populations of Arabidopsis that are important for adaptation to many different environments and are the result of many different evolutionary histories. These different environments will include different soil types, day lengths, pest populations, moisture levels, temperatures, and other factors. This will eventually lead to a better understanding of important adaptations to incorporate into the world's crops.