Arabidopsis 2010: A Systematic Approach to Automated Production of Recombinant Inbred Lines

该项目的目标是为科学界开发一种资源，促进自然遗传变异的扩大利用，以实现2010年项目的目标，即为每个拟南芥基因分配一个功能。至少四组新的重组自交系（RILs）将被制作出来。将系统筛选来自数百个可用野生型材料的潜在亲本对，以进行遗传和表型变异以及成对距离的筛选。选择的亲本将包括以前未开发的野生系。简单序列长度DNA多态性（SSLPs）将尽可能多地用于定位。来自400条RI线的95个个体将在100个位点上绘制地图，生成密度约为6厘米的地图。到2004年1月，所有RILs的种子将通过拟南芥生物资源中心（http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/ABRCHOME.HTM）公开，地图和制图数据将通过拟南芥信息资源（http://www.arabidopsis.org/）在线提供。在这个项目的过程中，本科生、研究生和技术人员将接受机器人、分子生物学、遗传学和数量性状制图方面的培训。此外，奥斯汀社区学院生物技术专业的学生将通过外展项目接受类似的培训。传统遗传学在少量实验室拟南芥品系中使用，将无法达到2010年项目的目标，即完全了解每个植物基因的功能。该项目将提供一种方法来绘制和鉴定当地野生拟南芥种群的基因，这些基因对于适应许多不同的环境非常重要，并且是许多不同进化历史的结果。这些不同的环境将包括不同的土壤类型、日照长度、害虫数量、湿度水平、温度和其他因素。这将最终使人们更好地了解重要的适应措施，并将其纳入世界作物中。

The goal of this project is to develop a resource for the scientific community that promotes expanded use of natural genetic variation in reaching the 2010 Project objective of assigning a function to each Arabidopsis gene. At least four new sets of mapped Recombinant Inbred Lines (RILs) will be produced. Potential pairs of parents from hundreds of available wild type accessions will be systematically screened for genetic and phenotypic variance and pairwise distances. The chosen parents will include wild lines not previously exploited. Simple Sequence Length DNA Polymorphisms (SSLPs) will be used as much as possible for mapping. Ninety-five individuals, from sets of 400 RI lines will be mapped at one hundred loci, generating a map with a density of approximately 6 centiMorgans. Seeds for all RILs will be publicly available through the Arabidopsis Biological Resource Center (http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/ABRCHOME.HTM) and maps and mapping data will be available online through The Arabidopsis Information Resource (http://www.arabidopsis.org/) by January 2004. During the course of this project, undergraduate and graduate students and technicians will be trained in robotics, molecular biology, genetics, and quantitative trait mapping. In addition, students in the Biotechnology Program at the Austin Community College will receive similar training through an outreach program. Conventional genetics used in a small number of laboratory strains of Arabidopsis, will not be able to reach the 2010 Project goal of complete knowledge of every plant gene's function. This project will provide a way to map and identify genes in local wild populations of Arabidopsis that are important for adaptation to many different environments and are the result of many different evolutionary histories. These different environments will include different soil types, day lengths, pest populations, moisture levels, temperatures, and other factors. This will eventually lead to a better understanding of important adaptations to incorporate into the world's crops.