Acquisition of a Quantitative Gene Detection and Expression System

获得定量基因检测和表达系统

• 批准号: 0420639
• 负责人: Brian Rogerson
• 金额: $ 10.39万
• 依托单位: Stockton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0420639&HistoricalAwards=false
• 关键词: Acquisition; Quantitative; Gene; Detection; Expression

A grant has been awarded to The Richard Stockton College of New Jersey under the direction of Dr. Brian Rogerson for the acquisition of a quantitative gene detection and expression system. This acquisition project will enhance the capabilities of our Core Molecular Analysis Facility, which is being used to support undergraduate student research, faculty research and teaching in the biology and chemistry programs, as well as collaborative research efforts with investigators outside the college. Research projects that will benefit from this technology include: (1) The examination of the Mycobacterium tuberculosis adaptive response to life in the lungs of infected mouse hosts exhibiting different levels of resistance (Dr. Rogerson), (2) the study of pollution effects on gene expression in winter flounder (Dr. Straub), a project that is being carried out in support of local fisheries research and that will compare individual fish from the highly pollution-impacted Raritan/Hudson Estuary with less impacted sites, and (3) the study of protein phosphatase 2A, a highly conserved cellular enzyme, and its role in regulating gene expression in the yeast Saccharomyces cerevisiae (Dr. York). This instrumentation will also allow gene expression experiments to be more easily integrated into upper level laboratory courses such as Biotechnology (Dr. Straub), Molecular Genetics (Dr. York) and Developmental Biology (Dr. Herlands). Addition of a quantitative sequence detection system to our undergraduate research laboratories is part of our continuing effort to provide relevant training opportunities for students that will prepare them for jobs in the technology sector or prepare them for graduate and professional training. This will help us fulfill one of the goals of the New Jersey Commission on Higher Education. This project is expected to contribute to our understanding of: (1) bacterial adaptation to the immune response in mammals, (2) biomarkers for surveying fish habitats for suitability, production or degree of contamination, and (3) how model organisms such as yeast can shed light on basic cellular processes such as gene regulation. This acquisition project will also help maintain our core facility as a focal point for collaboration with researchers at other institutions.

在Brian Rogerson博士的指导下，新泽西的Richard斯托克顿学院获得了一笔赠款，用于购买定量基因检测和表达系统。该收购项目将提高我们的核心分子分析设施的能力，该设施用于支持本科生研究，生物学和化学课程的教师研究和教学，以及与学院外的研究人员的合作研究工作。 将受益于这项技术的研究项目包括：（1）检测结核分枝杆菌对感染小鼠宿主肺中生命的适应性反应，所述小鼠宿主表现出不同水平的抗性（2）污染对比目鱼基因表达影响的研究斯特劳布博士），该项目正在进行，以支持当地渔业研究，并将比较个别鱼类从高度污染影响的拉里坦/哈德逊河口与受影响较小的网站，（3）研究蛋白磷酸酶2A（一种高度保守的细胞酶）及其在酿酒酵母（Saccharomycescerevisiae）（约克博士）中调节基因表达的作用。这种仪器也将允许基因表达实验更容易地整合到高级实验室课程，如生物技术（斯特劳布博士），分子遗传学（约克博士）和发育生物学（Herlands博士）。在我们的本科研究实验室中添加定量序列检测系统是我们不断努力的一部分，为学生提供相关的培训机会，为他们在技术领域的工作做好准备，或为他们的研究生和专业培训做好准备。这将有助于我们实现新泽西高等教育委员会的目标之一。该项目预计将有助于我们理解：（1）细菌对哺乳动物免疫反应的适应，（2）用于调查鱼类栖息地的适宜性，生产或污染程度的生物标志物，以及（3）酵母等模式生物如何揭示基因调控等基本细胞过程。这一收购项目还将有助于保持我们的核心设施作为与其他机构研究人员合作的焦点。