Acquisition of a Fluorescent Activated Cell Sorter (FACS) for SDSU Biology Department

在Kathleen McGuire博士的指导下，圣地亚哥州立大学获得了一笔赠款，用于通过购买荧光激活细胞分选仪或FACS，部分支持在SDSU建立流式细胞术设施。生物学的五个主要用户的研究目前需要使用流式细胞术，这是一种允许分析单个细胞的各种性状和特征的技术。一个足够复杂的仪器，以服务于教师在SDSU多年来将被购买和放置在生物系。 这台流式细胞仪将是SDSU的第一台同类仪器，也是唯一一台可用于该基金的五个合作PI提出的许多实验的仪器。生物学的其他几个学院也将使用该仪器，用于不同沙门氏菌菌株的宿主特异性，海鞘中notachords的发展以及骨骼和心肌结构和功能等不同的研究领域。该设施还将为化学系的教师和新成立的微生物研究中心的成员提供服务。该设施的两个独特的方面将是生态学家以及细胞和分子生物学家和生物化学家的使用，以及原核和真核细胞的使用。流式细胞仪设施还将允许广泛的本科生和研究生动手培训，并允许将流式细胞仪的使用纳入生态学、微生物学以及细胞和分子生物学的研究生和本科生课程。由于约44%的生物学专业和23%的SDSU的研究生属于代表性不足的少数群体，他们获得这一设施和仪器将很容易实现。 上述每个研究项目将通过在SDSU收购流式细胞仪设施得到显着增强，校园内的许多其他研究项目也是如此。SDSU的学生将首次有机会了解这一重要技术，并将能够获得技能，为他们提供更好的就业机会，并使他们能够以新的方式为大圣地亚哥地区和加州州的大型生物技术产业做出重大贡献。

A grant has been awarded to San Diego State University under the direction of Dr. Kathleen McGuire for partial support for the establishment of a flow cytometry facility at SDSU through the purchase of a fluorescence-activated cell sorter or FACS. The research of five major users in Biology currently requires the use of flow cytometry, a technology that allows single cells to be analyzed for a variety of traits and characteristics. An instrument that is sophisticated enough to serve the faculty at SDSU for many years to come will be purchased and placed in the Biology department. This flow cytometer will be the first instrument of its kind at SDSU and the only one available for many of the experiments proposed by the five co-PIs of the grant. Several other faculty in Biology will also use the instrument, for research areas as diverse as the host specificity of different strains of Salmonella, the development of notachords in Ascidians, and skeletal and heart muscle structure and function. The facility will also service faculty from the department of Chemistry and members of the newly established Center for Microbial Studies. Two unique aspects of this facility will be its use by ecologists as well as cell and molecular biologists and biochemists, and its use for both prokaryotic and eukaryotic cells. The flow cytometry facility will also permit extensive undergraduate and graduate student hands-on training and allow the use of the FACS to be incorporated into both the graduate and undergraduate curriculum in ecology, microbiology and cell and molecular biology. Because ~44% of Biology majors and 23% of SDSU's graduate students belong to underrepresented minority groups, their access to this facility and instrumentation will be readily achieved. Each of the research programs outlined above will be significantly enhanced by the acquisition of a flow cytometry facility at SDSU, as will many other research programs across campus. The students at SDSU will be offered, for the first time, opportunities to learn about this important technology and will be able to gain skills that will provide them with better job opportunities and allow them to significantly contribute in a new way to the large biotechnology industry in the greater San Diego area and the State of California.