SBIR Phase II: Rapid Detection of Bacterial Contaminants Using Micro-Fluidic Biochips

这一小型企业创新研究(SBIR)第二阶段项目将开发一个基于微流控的系统，使用细菌的介电浓度作为中间步骤来检测活的病原体。这个系统将使用第一级浓缩器，然后是介电浓缩，最后通过在带有集成阻抗测量的培养基中培养来检测培养物的生长。该项目的商业应用将是生物制药生产作业中的水媒微生物检测。与目前需要2-7天才能得出结果的技术不同，这种拟议的方法将在不到3小时的时间内对水样中的微生物进行电子检测。

This Small Business Innovation Research (SBIR) Phase II project will develop a microfluidic based system for the detection of viable pathogens using dielectric concentration of bacteria as an intermediate step. This system would use a first-stage concentrator, followed by dielectrophoretic concentration, and finally by culturing in media with integrated impedance measurements to detect culture growth. The commercial application of this project will be on the detection of waterborne microorganisms in biopharmaceutical manufacturing operations. The proposed method would electronically detect the viability of microorganisms in water samples in less than 3 hours, unlike the current technology that takes 2-7 days to yield results.