U.S.-India Cooperative Research: Molecular Mechanism of Vitreoscilla Hemoglobin (VHb) Function : Identification and Characterization of Cellular Partner(s) of VHb (s)

美印合作研究：玻璃颤菌血红蛋白 (VHb) 功能的分子机制：VHb 细胞伴侣的识别和表征

• 批准号: 0422555
• 负责人: Benjamin Stark
• 金额: $ 2.65万
• 依托单位: Illinois Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0422555&HistoricalAwards=false
• 关键词: U.S.; India; Cooperative; Research; Molecular

0422555Stark Description: This award supports cooperative research in molecular biochemistry between India and the U.S. Its title is: Molecular Mechanism of Vitreoscilla Hemoglobin (VHb) Function: Identification and Characterization of Cellular Partners of VHbs. Professors Benjamin Stark and Dale Webster, Illinois Institute of Technology, Chicago will collaborate with Professor Kanak Dikshit, Senior Scientist, Institute of Microbial Technology, Chandigarh. Hemoglobins are essential oxygen carrying proteins and microbial hemoglobins are important for optimal growth of certain organisms. Vitreoscilla hemoglobin, which has been used in genetic engineering, has resulted in the improved ability of organisms to acquire and utilize oxygen, and has enhanced their abilities to degrade certain toxic chemicals. The investigators will perform experiments to characterize and obtain understanding on the biochemical function of the "hemoglobin" of the bacterium Vitreoscilla and how, when introduced into a hemoglobin-less bacterium by genetic engineering, it changes the biochemical pathways of its new host's metabolism. Scope: This research is of great scientific and practical value and will contribute to fundamental knowledge. Greater understanding of the mechanisms by which VHb enhances cell growth will help advance technologies in which the use of VHb has been found to be beneficial. Chief among these is the US PIs work on enhancing bacterial degradation of aromatic compounds including those, which have environmental contaminants. The collaboration will exploit the complementary expertise of the two groups of investigators (biochemistry and physical chemistry of proteins on the US side and recombinant DNA technology on the Indian side). The researchers have an established collaboration and have worked successfully. This research and exchange of scientists will be mutually beneficial for graduate and undergraduate students at both institutions

0422555斯塔克描述：该奖项支持印度和美国之间的分子生物化学合作研究，其标题是：玻璃体血红蛋白（VHb）功能的分子机制：VHb的细胞伴侣的鉴定和表征。 芝加哥伊利诺伊理工学院的本杰明·斯塔克教授和戴尔·韦伯斯特教授将与昌迪加尔微生物技术研究所的高级科学家Kanak Dikshit教授合作。 血红蛋白是必需的携氧蛋白，微生物血红蛋白对于某些生物体的最佳生长是重要的。 已用于基因工程的玻璃体血红蛋白提高了生物体获取和利用氧气的能力，并增强了它们降解某些有毒化学物质的能力。 研究人员将进行实验，以表征和了解细菌Vitreplora的“血红蛋白”的生化功能，以及当通过基因工程引入血红蛋白较少的细菌时，它如何改变其新宿主的生化途径代谢。 研究范围：本研究具有重要的科学价值和实用价值，并将有助于基础知识。 更多地了解VHb促进细胞生长的机制将有助于推进已发现使用VHb有益的技术。 其中最主要的是美国PI致力于增强芳香族化合物的细菌降解，包括那些具有环境污染物的化合物。 这项合作将利用两组研究人员的互补专业知识（美国方面的蛋白质生物化学和物理化学，印度方面的重组DNA技术）。 研究人员建立了合作关系，并取得了成功。 这种研究和科学家的交流将对两个机构的研究生和本科生都有利