RUL1 to RUL3:Characterization of three novel genes from Arabidopsis thaliana

RUL1 至 RUL3：拟南芥三个新基因的表征

• 批准号: 168147811
• 负责人: Professor Dr. Norbert Sauer
• 金额: --
• 依托单位: Department Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/168147811?language=en
• 关键词: RUL1; RUL3; Characterization; three; novel

In a mutant screen for altered symplastic connectivity, a gene (RUG8) was identified that encodes a previously uncharacterized “protein of unknown function”. We showed that this protein is most likely a novel transcription factor that affects Arabidopsis root development, including the formation of the quiescent center, cellular maturation and radial patterning - but also the symplastic connectivity between root cells. The present proposal focuses on the characterization of three RUG8-like genes, RUL1 to RUL3 that show significant structural and sequence homologies to RUG8. Like RUG8, these genes encode previously uncharacterized proteins. All three RUL3 cDNAs and promoters were cloned, and preliminary analyses of available mutant lines were performed. As RUG8, RUL2 and RUL3 are nuclear localized, and first analyses of a rul3 mutant revealed phenotypic alterations (reduced unloading of GFP from the phloem and altered root development) that are similar to those found in rug8 mutants. The aim of the present project is the functional and physiological characterization of RUL1, RUL2 and RUL3.

在共质体连接性改变的突变体筛选中，鉴定了编码先前未表征的“未知功能的蛋白质”的基因（RUG 8）。我们发现，这种蛋白质很可能是一种新的转录因子，影响拟南芥根的发育，包括形成的静止中心，细胞成熟和径向图案-但也根细胞之间的共质体连接。目前的建议集中在三个RUG 8样基因，RUL 1至RUL 3，显示显着的结构和序列同源性RUG 8的表征。像RUG 8一样，这些基因编码以前未表征的蛋白质。克隆了所有三种RUL 3 cDNA和启动子，并对可用的突变株系进行了初步分析。由于RUG 8、RUL 2和RUL 3是核定位的，并且对RUL 3突变体的第一次分析揭示了与在rug 8突变体中发现的那些相似的表型改变（GFP从韧皮部的卸载减少和根发育改变）。本项目的目的是RUL 1，RUL 2和RUL 3的功能和生理特性。