Tissue- and organ specificity of jasmonate responses in the development of tomato flowers

番茄花发育过程中茉莉酸反应的组织和器官特异性

• 批准号: 168327665
• 负责人: Professorin Dr. Bettina Hause
• 金额: --
• 依托单位: Abteilung Stoffwechsel- und Zellbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/168327665?language=en
• 关键词: Tissue; organ; specificity; jasmonate; responses

Jasmonic acid (JA) and its derivatives, collectively known as jasmonates, are lipid-derived signaling molecules involved in defense against pathogens and in response to wounding by herbivores, in the regulation of mutualistic interactions of plants with microorganisms, but also in plant growth and development. Many of them alter gene expression positively or negatively in a regulatory network. Only few data exist on the function of JA in development of tomato flowers. Therefore, we plan to record during flower development: (i) the amount and the occurrence of bioactive jasmonates in various flower organs, and (ii) the jasmonaterelated gene expression and metabolite accumulation in female and male reproductive tissues. The following complementary approaches will be used: (a) detailed jasmonate profiling of dissected flower organs, (b) immuno cytochemical detection of jasmonates using a highly specific JA antibody to visualize bioactive jasmonates tissue specifically, and (c) transcript and metabolite profiling of reproductive tissues isolated from wild type and jai1, a mutant defective in JA perception, thereby known to exhibit defects in female development. To this end, we will identify JA-dependent regulatory components and metabolic pathways essential for proper development of reproductive organs in tomato.

茉莉酸（Jasmonic acid， JA）及其衍生物统称为茉莉酸盐，是一种脂质衍生的信号分子，参与防御病原体和对食草动物伤害的反应，调节植物与微生物的相互作用，也参与植物的生长发育。它们中的许多在调节网络中积极或消极地改变基因表达。目前关于JA在番茄花发育中的作用的研究资料很少。因此，我们计划记录在花发育过程中：(i)生物活性茉莉酸盐在花各器官中的数量和发生；（ii）茉莉酸盐相关基因在雌雄生殖组织中的表达和代谢物的积累。将使用以下补充方法：(a)解剖花器官的详细茉莉酸谱分析，(b)使用高度特异性的茉莉酸抗体对茉莉酸进行免疫细胞化学检测，以特异性地观察生物活性茉莉酸组织，以及(c)从野生型和jai1分离的生殖组织的转录物和代谢物谱分析，jai1是一种茉莉酸感知缺陷的突变体，因此已知在女性发育中表现出缺陷。为此，我们将确定番茄生殖器官正常发育所必需的ja依赖的调控成分和代谢途径。