Cell and tissue specific monitoring of jasmonates in plant interactions with pathogens and symbionts

植物与病原体和共生体相互作用中茉莉酸的细胞和组织特异性监测

• 批准号: 38642209
• 负责人: Professorin Dr. Bettina Hause
• 金额: --
• 依托单位: Abteilung Stoffwechsel- und Zellbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/38642209?language=en
• 关键词: Cell; tissue; specific; monitoring; jasmonates

Jasmonic acid (JA) and its derivatives collectively known as jasmonates belong to a family of lipid-derived signaling molecules that regulates many aspects of plant life, including defense against herbivores and pathogens, but also symbiotic interactions with mycorrhizal fungi. Jasmonates alter gene expression positively or negatively in a regulatory network. Most functions of jasmonates have been identified through analysis of mutants that are JA-insensitive or affected in the biosynthesis of JA. To broaden our knowledge on the mechanism of JA perception in response to pathogens and symbionts, the development but also the utilization of a detection system for JA at cell and tissue specific level are the main goals of this project. The approaches used for detection of JA in transgenic plants are complementary and will employ bimolecular fluorescent complementation (BiFC) using the in vivo interaction of COI1 with JAZ proteins as well as a synthetic JA-specific promoter fused to nuclear encoded GFP to visualize early and late JA-responsive cells, respectively. After isolation of nuclei from these cells by fluorescence-activated cell sorting (FACS), transcript profiling will be carried 2 out. Time-dependent and cell-specific patterns of gene expression as a read-out of cell-specific functions will lead to identification of new JA-responsive genes. Additionally, a screen for small noncoding RNAs regulating transcript levels of JA-responsive genes will be performed. At the end, the identification of new regulatory elements in JA-signaling is expected.

茉莉酸（JA）及其衍生物统称为茉莉酸酯属于脂质衍生的信号分子家族，调节植物生命的许多方面，包括防御食草动物和病原体，以及与菌根真菌的共生相互作用。茉莉酸在调控网络中积极或消极地改变基因表达。茉莉酸酯的大多数功能已经通过分析对JA不敏感或在JA生物合成中受影响的突变体来鉴定。为了拓宽我们对JA感知机制的认识，以应对病原体和共生体，JA在细胞和组织特异性水平上的检测系统的开发和利用是本项目的主要目标。用于检测转基因植物中JA的方法是互补的，并且将采用双分子荧光互补（BiFC），其使用COI 1与JAZ蛋白的体内相互作用以及与核编码的GFP融合的合成JA特异性启动子来分别可视化早期和晚期JA响应细胞。通过荧光激活细胞分选（FACS）从这些细胞中分离细胞核后，将进行转录谱分析。时间依赖性和细胞特异性的基因表达模式作为细胞特异性功能的读出将导致新的JA响应基因的鉴定。此外，还将筛选调节JA应答基因转录水平的小非编码RNA。最后，在JA-信号转导的新的调控元件的识别是预期的。