Interplay of auxin and jasmonates in tomato gynoecia development and fruit set

生长素和茉莉酸在番茄雌核发育和坐果中的相互作用

• 批准号: 404178704
• 负责人: Professorin Dr. Bettina Hause
• 金额: --
• 依托单位: Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/404178704?language=en
• 关键词: Interplay; auxin; jasmonates; tomato; gynoecia

Successful plant reproduction depends on tight coordination of flower-organ development, fertilization and fruit set. The plant hormones auxin and jasmonic acid (JA) play important roles in the timing and coordination of these processes. Our preliminary work suggested that auxin and JA interact in the regulation of gynoecia development and of the initiation of fruit set in response to fertilization. It also identified the transcription factors SlMYB21 and SlMYB24 as regulated by both auxin and JA. In the first period of the project, we identified the auxin-response factors SlARF8A and SlARF8B as central regulators of gynoecia development and fruit set. We found that Slarf8a Slarf8b double mutants are impaired in style and stigma development and produce seedless fruits independently of fertilization. Furthermore, gynoecia of Slarf8a Slarf8b mutant plants have reduced JA levels as well as SlMYB21 and SlMYB24 expression. We have generated mutants harboring a knockout of SlMYB24 and Slmyb21 Slmyb24 double mutant plants and found that Slmyb24 substantially enhances the Slmyb21 phenotype with respect to gynoecia development and fruit set. These results suggest that SlARF8, SlMYB21/24 and JA interact to regulate flower and fruit development. In the renewal of the proposal, we aim to address the following hypotheses: (i) SlARF8 promotes JA production, possibly by activating SlMYB21, and SlMYB21 inhibits SlARF8 expression; (ii) SlMYB21 and SlMYB24 act together to regulate flower development and the transition to fruit set; and (iii) SlARF8, SlMYB21/24 and JA are central regulators of stigma development. We will test these hypotheses by examining the genetic and molecular interactions among SlARF8, SlMYB21/24 and JA in gynoecia development and particularly stigma development, and in fruit set. We will: 1. Elucidate the regulatory interaction between SlMYB21 and SlARFs by generation and characterization of double and triple mutants. 2. Characterize the effect of simultaneous loss of function of both SlMYB21 and SlMYB24. 3. Characterize stigma development and gene expression in wild type and mutant plants. The research is expected to provide insights on the coordinated function of MYBs and ARFs in tomato reproduction and will shed light on the crosstalk between auxin and JA in the regulation of reproduction in tomato.

植物的成功繁殖依赖于花器官发育、受精和坐果的紧密协调。植物激素生长素和茉莉酸（JA）在这些过程的定时和协调中起着重要作用。我们的初步研究表明，生长素和JA在受精反应中共同调控雌蕊发育和坐果形成。还鉴定了受生长素和JA共同调控的转录因子SlMYB21和SlMYB24。在项目的第一期，我们确定了生长素反应因子SlARF8A和SlARF8B是雌蕊发育和结实的主要调控因子。我们发现Slarf8a和Slarf8b双突变体花柱和柱头发育受损，不受受精影响而产生无籽果实。此外，Slarf8a和Slarf8b突变株的雌蕊中JA水平降低，SlMYB21和SlMYB24的表达也降低。我们已经产生了含有敲除SlMYB24和Slmyb21的突变体，并发现SlMYB24在雌蕊发育和结实方面显著增强了Slmyb21的表型。这些结果表明，SlARF8、SlMYB21/24和JA相互作用调控了花和果实的发育。在更新的建议中，我们的目标是解决以下假设：(i) SlARF8可能通过激活SlMYB21促进JA的产生，而SlMYB21抑制SlARF8的表达；（ii） SlMYB21和SlMYB24共同调控花发育和向坐果过渡；（iii） SlARF8、SlMYB21/24和JA是柱头发育的主要调控因子。我们将通过研究SlARF8、SlMYB21/24和JA在雌蕊发育，特别是柱头发育和坐果过程中的遗传和分子相互作用来验证这些假设。我们将：1；通过双突变体和三突变体的产生和表征来阐明SlMYB21和SlARFs之间的调控相互作用。2. 描述SlMYB21和SlMYB24同时丧失功能的影响。3. 描述野生型和突变型植物柱头发育和基因表达。该研究将进一步揭示MYBs和ARFs在番茄繁殖中的协同作用，并揭示生长素和JA在番茄繁殖调控中的串扰关系。